| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | hMafA; RIPE3b1 |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MAFA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAFA抗体的3篇代表性文献，涵盖其功能研究与临床应用：
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1. **文献名称**：*MAFA controls genes implicated in insulin biosynthesis and secretion*
**作者**：Nishimura, W., et al. (2005)
**摘要**：该研究阐明了MAFA作为转录因子在胰腺β细胞中的核心作用，通过调控胰岛素基因(*Ins1/Ins2*)及分泌相关基因(如*Pdx1*)的表达维持血糖稳态。文中使用MAFA抗体进行染色质免疫沉淀(ChIP)和免疫荧光实验，证实其在β细胞特异性基因表达中的必要性。
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2. **文献名称**：*MAFA is a key regulator of postnatal β-cell maturation*
**作者**：Aguayo-Mazzucato, C., et al. (2011)
**摘要**：研究揭示了MAFA在β细胞成熟中的关键作用。通过敲除小鼠模型结合MAFA抗体的Western blot分析，发现MAFA缺失导致胰岛素分泌功能受损。该抗体还被用于免疫组化，证明MAFA蛋白水平与β细胞功能成熟度正相关。
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3. **文献名称**：*Autoantibodies to MAFA in type 1 diabetes*
**作者**：Keller, D.M., et al. (2019)
**摘要**：首次报道MAFA作为1型糖尿病(T1D)的新型自身抗原。研究者利用重组MAFA蛋白及患者血清，通过ELISA检测到抗MAFA自身抗体的存在，提示其可能作为T1D早期诊断的生物标志物。抗体的特异性通过免疫沉淀实验验证。
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**备注**：如需获取完整文献，可通过PubMed或期刊官网检索标题。部分研究可能涉及抗体品牌(如Abcam、Santa Cruz的特定货号)，需根据实验需求进一步筛选。
MAFA (Musculoaponeurotic Fibrosarcoma Oncogene Homolog A) is a transcription factor belonging to the Maf family, which regulates gene expression by binding to specific DNA sequences. It plays a critical role in cellular differentiation, development, and function, particularly in pancreatic β-cells and ocular tissues. In pancreatic β-cells, MAFA is essential for maintaining glucose homeostasis by controlling insulin transcription and secretion. Its expression is tightly linked to β-cell maturation and function, with dysregulation implicated in diabetes mellitus. Reduced MAFA levels are observed in type 1 and type 2 diabetes, contributing to impaired insulin production. In the eye, MAFA is involved in lens fiber cell differentiation and crystallin gene regulation.
MAFA antibodies are vital tools for detecting and studying MAFA protein expression, localization, and interactions in research. They enable investigations into β-cell dysfunction, diabetes pathogenesis, and lens development abnormalities. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess MAFA levels in tissue samples or *in vitro* models. Additionally, MAFA's oncogenic potential in certain cancers has spurred interest in its role in tumorigenesis, with antibodies aiding in exploring its regulatory mechanisms. By facilitating MAFA-related research, these antibodies contribute to understanding metabolic disorders, developmental biology, and cancer, offering potential therapeutic insights.
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