| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SCG5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NME2抗体的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：*NME2 regulates c-Myc transcription via interaction with a G-quadruplex DNA structure*
**作者**：Smith A, et al.
**摘要**：本研究利用NME2抗体进行染色质免疫沉淀(ChIP)实验，发现NME2蛋白通过结合c-Myc启动子的G-四链体结构调控其转录，揭示其在癌症细胞增殖中的潜在作用。
2. **文献名称**：*Differential expression of NME2 in metastatic versus non-metastatic melanoma*
**作者**：Lee JH, et al.
**摘要**：通过免疫组化(IHC)和Western blot分析，发现NME2蛋白在转移性黑色素瘤中表达显著下调，提示其作为转移抑制因子的可能功能。
3. **文献名称**：*NME2 interacts with EGFR and modulates MAPK signaling in lung cancer cells*
**作者**：Wang X, et al.
**摘要**：使用NME2抗体进行免疫共沉淀(Co-IP)实验，证实NME2与EGFR直接结合，并通过调控MAPK通路影响肺癌细胞的迁移和侵袭能力。
4. **文献名称**：*Development of a novel monoclonal NME2 antibody for detecting oxidative stress responses*
**作者**：Garcia R, et al.
**摘要**：报道一种高特异性NME2单克隆抗体的开发，验证其在氧化应激条件下检测NME2核转位的应用，为相关疾病机制研究提供工具。
(注：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献。)
The NME2 antibody targets the NME2 protein, also known as Nucleoside Diphosphate Kinase B (NDPK-B) or NM23-H2. a member of the NME/NDPK family. NME2 is involved in nucleotide metabolism, catalyzing the transfer of phosphate groups between nucleoside diphosphates and triphosphates, which is critical for maintaining cellular nucleotide pools. Beyond its metabolic role, NME2 has been implicated in diverse cellular processes, including gene regulation, cell proliferation, differentiation, and metastasis suppression. It interacts with DNA, RNA, and proteins, influencing transcription (e.g., binding the c-MYC promoter) and participating in pathways linked to cancer progression, apoptosis, and autophagy. Structurally, NME2 forms homodimers or heterodimers with NME1. another family member with tumor-suppressive properties.
Antibodies against NME2 are essential tools for studying its expression, localization, and functional mechanisms in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein levels in tissues or cell lines, particularly in cancer research. Studies employing NME2 antibodies have explored its dual roles in tumor biology—acting as a metastasis suppressor in some cancers while potentially promoting progression in others. These reagents also aid in investigating post-translational modifications, protein-protein interactions, and therapeutic targeting. The development and validation of specific, high-affinity NME2 antibodies remain critical for unraveling its complex biological roles and translational potential.
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