| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AIP1; ALIX; HP95; DRIP4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PDCD6IP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇涉及PDCD6IP(ALIX)抗体的代表性文献摘要：
1. **文献名称**：*"ALIX regulates tumor-mediated immunosuppression by controlling EGFR activity and PD-L1 presentation"*
**作者**：Zhang X, et al.
**摘要**：该研究利用PDCD6IP/ALIX特异性抗体，通过免疫沉淀和Western blot技术，揭示了ALIX通过调控EGFR内吞和PD-L1的细胞外囊泡分泌，影响肿瘤免疫逃逸的分子机制。
2. **文献名称**：*"Structural basis of PDCD6IP/ALIX interaction with HIV-1 late domain"*
**作者**：Fisher RD, et al.
**摘要**：通过晶体结构分析和抗体共沉淀实验，阐明ALIX与HIV-1病毒颗粒释放相关的p6结构域结合模式，为抗病毒靶点开发提供理论依据。
3. **文献名称**：*"PDCD6IP/ALIX modulates exosomal miRNA sorting and cancer cell communication"*
**作者**：Baietti MF, et al.
**摘要**：使用PDCD6IP抗体阻断实验，证明ALIX通过ESCRT通路调控外泌体miRNA分选，影响肿瘤微环境中细胞间通讯，促进转移前微环境形成。
注：以上为模拟文献摘要，实际引用时请通过PubMed等平台核对原文信息。建议优先选择近3-5年发表于*Nature Cell Biology*、*Cell Research*等期刊的原始研究论文。
**Background of PDCD6IP Antibody**
PDCD6IP (Programmed Cell Death 6 Interacting Protein), also known as ALIX, is a multifunctional adaptor protein involved in apoptosis, endocytosis, and extracellular vesicle biogenesis. It plays a critical role in the endosomal sorting complex required for transport (ESCRT) pathway, mediating membrane remodeling events such as cytokinesis, viral budding, and exosome formation. ALIX interacts with components of the ESCRT machinery (e.g., TSG101. CHMP4) and apoptotic proteins like PDCD6 (ALG-2), linking cellular processes like programmed cell death and membrane dynamics.
PDCD6IP antibodies are essential tools for studying its diverse functions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect ALIX expression, localization, and protein interactions. These antibodies help elucidate its role in diseases, including cancer (e.g., regulating tumor suppression or metastasis), neurodegenerative disorders (e.g., modulating exosome-mediated pathogenic protein spread), and viral infections (e.g., HIV-1 budding). Researchers also utilize PDCD6IP antibodies to explore its structural domains, such as the Bro1 domain (binding ESCRT-III) and the V domain (interacting with viral proteins), providing insights into therapeutic targeting of ALIX-associated pathways.
×
