| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CMG1; CCDC2; CMG-1 |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human IFT74 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是几篇可能与IFT74抗体相关的参考文献示例(注：以下为模拟构造的示例，实际文献需通过学术数据库查询确认)：
1. **文献名称**: *"IFT74 is essential for ciliary transport and maintenance in mammalian cells"*
**作者**: Smith J, et al.
**摘要**: 研究利用IFT74特异性抗体通过免疫荧光和Western blot分析，证实IFT74在纤毛组装中的关键作用，敲低后导致纤毛结构异常，提示其在运输复合体中的功能。
2. **文献名称**: *"Characterization of IFT complex B proteins in polycystic kidney disease models"*
**作者**: Lee H, Brown K.
**摘要**: 通过IFT74抗体检测多囊肾模型小鼠肾组织中的表达变化，发现IFT74与纤毛信号传导异常相关，抗体染色显示其在囊肿形成区域的定位紊乱。
3. **文献名称**: *"Functional analysis of IFT74 mutations in zebrafish ciliopathy models"*
**作者**: Chen R, et al.
**摘要**: 在斑马鱼中利用抗IFT74抗体进行免疫组化，揭示突变体胚胎中纤毛蛋白运输缺陷，证实IFT74在发育中的保守性及与人类纤毛疾病的关联。
4. **文献名称**: *"Proteomic profiling of IFT-B subunits in primary cilia"*
**作者**: Gonzalez M, et al.
**摘要**: 结合质谱分析与IFT74抗体验证，系统鉴定了IFT-B复合体成员在纤毛中的相互作用网络，强调IFT74在复合体稳定性中的角色。
**建议**：实际文献可通过PubMed或Google Scholar检索关键词“IFT74 antibody”、“IFT74 cilia”获取，部分研究可能涉及抗体应用(如货号HPA123456的商业抗体)。
The IFT74 antibody is a crucial tool for studying the intraflagellar transport (IFT) system, essential for the assembly and maintenance of cilia and flagella. IFT74 is a core component of the IFT-B complex, which mediates anterograde transport of cargo from the base to the tip of cilia. This protein plays a vital role in ciliogenesis, signaling pathways (e.g., Hedgehog), and sensory functions. Mutations in IFT74 are linked to ciliopathies, a group of disorders affecting multiple organs, including retinal degeneration, renal abnormalities, and skeletal defects.
Researchers use IFT74 antibodies primarily in techniques like Western blotting, immunofluorescence, and immunohistochemistry to visualize protein expression, localization, and dynamics in ciliated cells or tissues. These antibodies help elucidate ciliary defects in disease models and assess IFT complex interactions. Commercial IFT74 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation includes testing in knockout controls or siRNA-treated samples to confirm specificity.
Studies employing IFT74 antibodies have advanced understanding of ciliary biology, contributing to therapeutic insights for ciliopathies and cancer, where disrupted cilia are implicated in tumor progression and metastasis.
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