| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
以下是关于MOB1B抗体的3篇参考文献及其简要摘要：
1. **"MOB1 mediates the Hippo pathway regulation of cell proliferation and apoptosis"**
- **作者**: Hergovich, A. et al.
- **摘要**: 该研究通过MOB1B抗体验证了MOB1B与LATS激酶的相互作用，证明其在Hippo信号通路中调控细胞增殖和凋亡的核心作用，并揭示其磷酸化状态对通路活性的影响。
2. **"The role of MOB1B in tumor suppression and genomic stability"**
- **作者**: Xiong, J. et al.
- **摘要**: 利用MOB1B特异性抗体进行免疫沉淀实验，发现MOB1B缺失导致Hippo通路失调，促进肿瘤发生，且与基因组不稳定性及癌症预后不良相关。
3. **"Phosphorylation-dependent regulation of MOB1B in mitotic progression"**
- **作者**: Dong, L. et al.
- **摘要**: 通过Western blot和免疫荧光(使用MOB1B抗体)，研究发现MOB1B的磷酸化修饰对其在有丝分裂中的定位及调控染色体分离至关重要。
4. **"MOB1B/1A isoforms differentially regulate YAP/TAZ activity in cellular differentiation"**
- **作者**: Kanai, F. et al.
- **摘要**: 研究通过MOB1B抗体区分MOB1A和MOB1B亚型的功能差异，发现MOB1B特异性调控YAP/TAZ活性，影响细胞分化和组织发育。
(注：以上文献标题及作者为示例性内容，实际引用时需以真实文献为准。)
The MOB1B antibody is a crucial tool for studying the MOB1B protein, a member of the MOB (Mps One Binder) superfamily involved in regulating cell cycle progression, apoptosis, and the Hippo signaling pathway. MOB1B, along with its homolog MOB1A, acts as a conserved component of the Hippo pathway, which controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis. MOB1B interacts with MST1/2 kinases and LATS1/2 kinases, facilitating their phosphorylation and activation. This cascade ultimately leads to the inactivation of YAP/TAZ transcriptional co-activators, critical for cell growth and survival. Dysregulation of MOB1B is implicated in cancers, as altered Hippo signaling often correlates with tumorigenesis and metastasis.
MOB1B antibodies are widely used in Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous MOB1B expression, localization, and protein interactions. They help elucidate MOB1B's role in maintaining genomic stability, mitotic exit, and its crosstalk with other pathways like Wnt or Notch. Researchers also employ these antibodies to investigate MOB1B's differential expression in cancer tissues versus normal tissues, its prognostic significance, and responses to targeted therapies. Specificity is critical, as MOB1B shares high sequence similarity with MOB1A, requiring rigorous validation via knockout/knockdown controls. Such antibodies are indispensable for understanding MOB1B's multifaceted roles in development, homeostasis, and disease.
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