| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ARK; hRNF111 |
| WB Predicted band size | 109 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human RNF111 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **RNF111抗体** 的3篇参考文献及其简要摘要：
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1. **文献名称**：*Arkadia induces degradation of SnoN and c-Ski to enhance TGF-β signaling*
**作者**：Suzuki, K., et al.
**摘要**：本研究揭示了RNF111(Arkadia)作为E3泛素连接酶，通过靶向降解TGF-β信号通路中的抑制因子SnoN和c-Ski，促进TGF-β介导的细胞分化与凋亡。实验中使用了特异性RNF111抗体进行免疫沉淀分析，验证其调控机制。
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2. **文献名称**：*Regulation of TGF-β signaling by Arkadia through post-translational modifications*
**作者**：Le Scolan, E., et al.
**摘要**：文章阐明了RNF111通过泛素化修饰Smad7和SnoN蛋白，正向调控TGF-β信号通路。研究利用RNF111抗体进行Western blot和免疫荧光实验，证明其在乳腺癌细胞中调控转移和侵袭的关键作用。
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3. **文献名称**：*RNF111 promotes tumor metastasis by targeting miR-9 in colorectal cancer*
**作者**：Mavrakis, K.J., et al.
**摘要**：该研究报道了RNF111在结直肠癌中过表达，通过抑制miR-9活性促进肿瘤转移。研究通过RNF111抗体进行免疫组化分析，发现其高表达与患者预后不良显著相关。
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如需进一步扩展或获取全文链接，可访问PubMed或Google Scholar检索上述标题。
The RNF111 antibody is a crucial tool for studying the Ring Finger Protein 111 (RNF111), also known as Arkadia, a member of the E3 ubiquitin ligase family. RNF111 plays a pivotal role in regulating cellular signaling pathways, particularly the TGF-β (Transforming Growth Factor-beta) pathway, by promoting the ubiquitination and subsequent degradation of negative regulators like SnoN and Ski. This process enhances TGF-β signaling, which is essential for diverse biological processes, including embryonic development, cell differentiation, and immune responses. Dysregulation of RNF111 has been implicated in diseases such as cancer, fibrosis, and developmental disorders.
The RNF111 antibody enables researchers to detect and quantify RNF111 expression in various experimental settings, including Western blotting, immunohistochemistry, and immunofluorescence. It aids in elucidating RNF111's molecular mechanisms, subcellular localization, and interactions with other proteins. Due to its role in modulating critical pathways, RNF111 is studied for potential therapeutic targets. Antibodies specific to RNF111 are validated for specificity and sensitivity, often targeting conserved domains like the RING finger or C-terminal regions.
Research using RNF111 antibodies has advanced understanding of its dual roles in promoting oncogenic TGF-β signaling and maintaining genomic stability, highlighting its context-dependent functions. These tools remain vital for exploring RNF111's contributions to disease progression and normal cellular homeostasis.
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