| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L28 |
| WB Predicted band size | 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPL28 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RPL28抗体的参考文献示例(内容为模拟，仅供参考)：
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1. **"RPL28 as a prognostic biomarker in colorectal cancer: Immunohistochemical analysis"**
*Authors: Zhang Y, et al. (2022)*
**摘要**：本研究通过免疫组化技术，利用RPL28抗体检测结直肠癌组织中RPL28蛋白表达水平，发现其高表达与患者不良预后显著相关，提示RPL28可能作为潜在治疗靶点。
2. **"RPL28 regulates hepatitis C virus replication via ribosome-mediated mechanisms"**
*Authors: Smith J, et al. (2018)*
**摘要**：文章通过RNA干扰和RPL28抗体敲低实验，证实RPL28与丙型肝炎病毒(HCV)RNA结合，调控病毒蛋白翻译及复制，为抗病毒策略提供新方向。
3. **"Developmental expression profiling of RPL28 in zebrafish embryos using specific antibodies"**
*Authors: Chen L, et al. (2016)*
**摘要**：利用RPL28抗体的Western blot及免疫荧光技术，分析斑马鱼胚胎发育中RPL28的动态表达，揭示其在神经管形成中的关键作用。
4. **"Validation of RPL28 antibody specificity for ribosome profiling studies"**
*Authors: Garcia R, et al. (2020)*
**摘要**：系统评估商业化RPL28抗体的特异性，通过免疫共沉淀和质谱验证其在核糖体蛋白复合物研究中的可靠性，为相关实验提供技术参考。
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(注：以上文献为模拟生成，实际引用需查询真实数据库如PubMed、Web of Science等。)
The RPL28 antibody targets ribosomal protein L28 (RPL28), a component of the 60S subunit of eukaryotic ribosomes. Ribosomal proteins like RPL28 are essential for protein synthesis, playing structural and functional roles in ribosome assembly and mRNA translation. RPL28. encoded by the RPL28 gene in humans, contains conserved domains that facilitate interactions with rRNA and other ribosomal proteins. Beyond its canonical role, emerging studies suggest ribosomal proteins, including RPL28. may participate in extraribosomal processes such as DNA repair, apoptosis regulation, and cellular stress responses.
RPL28 antibodies are widely used in research to study ribosome biogenesis, translational control, and dysregulation in diseases. These antibodies enable detection and localization of RPL28 via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific applications include investigating RPL28 expression variations in cancers, developmental disorders, or conditions linked to ribosomopathies—diseases caused by impaired ribosome function. For example, altered RPL28 levels have been observed in certain tumors, potentially influencing cell proliferation.
Commercially available RPL28 antibodies are typically raised in hosts like rabbits or mice, with validation in model organisms (e.g., human, mouse, rat). Researchers must verify antibody specificity using knockout controls or siRNA knockdowns to minimize cross-reactivity, given the high homology among ribosomal protein family members. Overall, RPL28 antibodies serve as critical tools for dissecting both ribosomal and non-ribosomal functions of this protein in health and disease.
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