| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | S12 |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPS12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RPS12抗体的3篇代表性文献，按研究主题分类整理：
1. **文献名称**：*RPS12 as a Novel Prognostic Biomarker in Lung Adenocarcinoma*
**作者**：Chen et al. (2022)
**摘要**：本研究通过免疫组化(使用兔源RPS12多克隆抗体)分析肺癌组织中RPS12蛋白表达水平，发现RPS12高表达与患者总生存期缩短显著相关，提示其可作为肺癌预后标志物。
2. **文献名称**：*Development of a Monoclonal Antibody Against Human RPS12 for Ribosome Profiling*
**作者**：Tanaka et al. (2020)
**摘要**：团队成功制备鼠源抗人RPS12单克隆抗体，经Western blot和免疫荧光验证特异性，应用于核糖体新生肽链复合物分析，为翻译调控研究提供工具。
3. **文献名称**：*RPS12 Dysregulation in Alzheimer's Disease Cortical Neurons*
**作者**：Smith et al. (2018)
**摘要**：利用商业化山羊抗RPS12抗体检测阿尔茨海默病患者脑组织，发现皮层神经元中RPS12蛋白异常聚集，可能与tau蛋白病理进程存在关联。
注：以上文献信息为示例性质，实际检索时建议通过PubMed或Web of Science以"RPS12 antibody"+"specific application"为关键词筛选近期实验性研究论文。真实文献中抗体应用多作为实验方法部分出现，专门针对抗体开发的文献相对较少。
The ribosomal protein S12 (RPS12) is a component of the 40S subunit of eukaryotic ribosomes, playing a critical role in protein synthesis by ensuring translational accuracy and ribosome stability. As part of the small ribosomal subunit, RPS12 interacts with mRNA and tRNA during initiation and elongation phases, contributing to codon-anticodon recognition. Beyond its canonical role, emerging studies suggest RPS12 may have extraribosomal functions, including involvement in cell proliferation, apoptosis, and DNA damage response pathways.
Antibodies targeting RPS12 are valuable tools for investigating ribosome biogenesis, translational regulation, and disease mechanisms. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study RPS12 expression, localization, and protein interactions. Dysregulation of RPS12 has been implicated in cancers (e.g., hepatocellular carcinoma, leukemia) and neurological disorders, with some studies highlighting its potential as a diagnostic or prognostic biomarker. Research also explores its role in viral infections, as certain viruses hijack ribosomal proteins for replication. Commercial RPS12 antibodies are typically raised against conserved epitopes, offering cross-reactivity across human, mouse, and rat samples. Validation often includes knockout cell lines to confirm specificity. Ongoing research continues to unravel RPS12's multifaceted roles in health and disease, driving demand for reliable antibodies in both basic and translational studies.
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