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Rabbit Polyclonal POLR2I Antibody

  • 中文名: POLR2I抗体
  • 别    名: RPB9; hRPB14.5
货号: IPDX02816
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesRPB9; hRPB14.5
WB Predicted band size15 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human POLR2I
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于POLR2I抗体的3篇参考文献(注:文献信息为模拟概括,建议通过学术数据库核对原文):

1. **文献名称**:*Structural insights into RNA polymerase II elongation complex stability*

**作者**:Cramer P. et al.

**摘要**:研究利用POLR2I抗体进行免疫沉淀,分析了RNA聚合酶II的亚基组成及其在转录延伸过程中的构象变化,揭示了POLR2I在维持复合物稳定性中的作用。

2. **文献名称**:*Dysregulation of RNA polymerase II subunits in cancer progression*

**作者**:Smith J.R., Wang L.

**摘要**:通过Western blot和免疫组化实验(使用POLR2I抗体),发现POLR2I在多种肿瘤组织中表达异常,提示其可能作为癌症诊断的生物标志物。

3. **文献名称**:*Antibody-based profiling of RNA polymerase II post-translational modifications*

**作者**:Nguyen T.H. et al.

**摘要**:开发了一种针对POLR2I磷酸化表位的特异性抗体,用于ChIP-seq分析,揭示了该亚基在基因启动子区域的特异性修饰模式及其对转录调控的影响。

**注意**:以上为示例性内容,实际文献需通过PubMed或Google Scholar检索关键词“POLR2I antibody”“RNA polymerase II RPB9”获取。

背景信息

The POLR2I antibody targets the POLR2I protein, a subunit of RNA polymerase II (Pol II), which is essential for eukaryotic transcription. POLR2I, also known as RPB9. plays a critical role in transcription initiation and elongation by contributing to the structural integrity of Pol II and participating in promoter recognition. This subunit is implicated in transcription-coupled DNA repair and may influence transcriptional fidelity by aiding in proofreading during RNA synthesis. Antibodies against POLR2I are widely used in research to study Pol II assembly, transcriptional regulation, and its dysregulation in diseases like cancer or neurodegenerative disorders. They enable detection of POLR2I expression and localization via techniques such as Western blotting, immunofluorescence, and ChIP-seq. POLR2I antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Commercial antibodies are typically raised in rabbits or mice, with applications spanning basic molecular biology and clinical studies exploring transcriptional anomalies. Understanding POLR2I's function through these antibodies provides insights into gene expression mechanisms and potential therapeutic targets.

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