| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | S25 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPS25 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与RPS25抗体相关的模拟参考文献(注：部分内容基于真实研究背景，但文献标题和作者为虚拟示例，建议通过学术数据库核实具体文献)：
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1. **标题**: *RPS25 modulates HIV-1 replication by interacting with viral RNA*
**作者**: Smith J, et al.
**摘要**: 研究利用RPS25抗体进行免疫沉淀和基因敲低实验，发现RPS25通过与HIV-1 RNA特异性结合，促进病毒翻译和组装，提示其作为抗病毒治疗靶点的潜力。
2. **标题**: *Ribosomal protein S25 depletion induces p53-dependent cell cycle arrest*
**作者**: Chen L, et al.
**摘要**: 通过RPS25抗体敲低细胞中RPS25表达，发现其缺失激活p53通路，导致细胞周期停滞和凋亡，揭示了核糖体蛋白在肿瘤抑制中的非经典功能。
3. **标题**: *Antibody-based profiling of RPS25 in hepatocellular carcinoma*
**作者**: Wang Y, et al.
**摘要**: 利用RPS25抗体对肝癌组织进行免疫组化分析，发现RPS25高表达与患者预后不良相关，可能通过调控Wnt/β-catenin通路促进肿瘤进展。
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**提示**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以“RPS25 antibody”或“RPS25 function”为关键词检索。推荐关注RPS25在病毒学(如HIV、HCV)、癌症及核糖体胁迫领域的研究。
**Background of RPS25 Antibody**
The RPS25 antibody is a tool designed to detect ribosomal protein S25 (RPS25), a component of the 40S subunit of eukaryotic ribosomes. RPS25 plays a critical role in mRNA translation, particularly in maintaining ribosome stability and ensuring accurate initiation and elongation processes. Beyond its canonical role in protein synthesis, RPS25 has been implicated in non-ribosomal functions, including viral RNA recruitment during infections (e.g., hepatitis C virus) and potential roles in cellular stress responses.
Research using RPS25 antibodies has expanded due to emerging interest in ribosome heterogeneity and its implications in disease. For example, altered RPS25 expression has been observed in certain cancers and neurodegenerative disorders, suggesting its involvement in pathological mechanisms. The antibody is widely utilized in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study RPS25 localization, expression levels, and interactions in various biological contexts.
Validated RPS25 antibodies are essential for specificity, as cross-reactivity with other ribosomal proteins can complicate interpretations. Many commercially available antibodies are raised against conserved peptide regions, often tested in knockout models to confirm target specificity. Recent studies also explore RPS25's role in selective mRNA translation and its potential as a therapeutic target, underscoring the antibody's relevance in both basic and translational research.
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