| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | alpha-fetoprotein; FETA; HPAFP |
| Entrez GeneID | 174 |
| clone | 6E6 |
| WB Predicted band size | 69kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human AFP expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于M1AP抗体的3篇虚构参考文献示例，供参考：
1. **文献名称**：*M1AP Antibody Development and Its Role in Meiotic Chromosome Segregation*
**作者**：Smith, J. et al.
**摘要**：本研究开发了一种高特异性M1AP多克隆抗体，通过免疫印迹和免疫荧光技术证实其在哺乳动物生殖细胞中的表达。实验表明，M1AP蛋白在减数分裂期参与染色体分离调控，抗体阻断实验导致小鼠生殖细胞分裂异常。
2. **文献名称**：*M1AP as a Novel Biomarker in Testicular Germ Cell Tumors: Immunohistochemical Analysis*
**作者**：Zhang, L. et al.
**摘要**：利用商业化M1AP单克隆抗体对睾丸生殖细胞肿瘤组织进行免疫组化分析，发现M1AP在精原细胞瘤中高表达，而在非生殖细胞肿瘤中缺失。研究提示M1AP抗体可作为鉴别诊断的辅助工具。
3. **文献名称**：*Functional Characterization of M1AP-Knockout Mice Using Targeted Antibody Probes*
**作者**：Tanaka, K. et al.
**摘要**：通过CRISPR技术构建M1AP基因敲除小鼠模型，采用定制抗体检测蛋白表达缺失。表型分析显示突变体雄性小鼠不育，证实M1AP在精子形成中不可或缺，抗体染色揭示其在精子头部形态发生中的作用。
(注：以上文献为示例性内容，实际研究中请以真实数据库检索结果为准。)
M1AP (Male Germ Cell-Associated Antigen 1) is a protein encoded by the M1AP gene, primarily expressed in germ cells during spermatogenesis. It plays a critical role in male meiosis, particularly in chromosome segregation and synapsis. Dysregulation of M1AP has been linked to infertility and germ cell tumorigenesis. Structurally, M1AP interacts with the meiosis-specific protein MEIOB, forming a complex essential for DNA double-strand break repair and homologous recombination.
M1AP antibodies are valuable tools in reproductive biology and oncology research. They enable the detection of M1AP protein expression in testicular tissues, aiding studies on spermatogenic failure and meiotic defects. In cancer research, M1AP antibodies help identify germ cell tumors (e.g., seminomas, nonseminomas) and certain somatic cancers that aberrantly express germ cell-specific antigens. Elevated M1AP levels in tumors may correlate with malignancy progression, making it a potential biomarker.
Recent studies also explore M1AP's role in mitotic errors and genomic instability in somatic cells, suggesting broader implications in carcinogenesis. Commercial M1AP antibodies are typically developed in rabbits or mice using recombinant protein epitopes, validated for applications like Western blot, immunohistochemistry, and immunofluorescence. Ongoing research focuses on its therapeutic targeting and diagnostic utility in infertility and oncology.
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