| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CTS; CTS1; PALB; TBPA; HEL111; HsT2651 |
| Entrez GeneID | 7276 |
| clone | 2E10C5 |
| WB Predicted band size | 15.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TTR (AA: 1-147) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **"THUMPD3-mediated tRNA m7G modification regulates cell cycle progression"**
*Authors: Li, X., Zhang, Y., & Wang, J.*
**摘要**：本研究揭示了THUMPD3通过催化tRNA的m7G甲基化修饰调控细胞周期进程的机制。利用特异性THUMPD3抗体进行RNA免疫沉淀(RIP)和蛋白质定位实验，发现其缺失导致G1/S期阻滞，并影响癌症细胞增殖。
2. **"THUMPD3 interacts with the RNA exosome to regulate ribosomal RNA processing"**
*Authors: Smith, R.P., et al.*
**摘要**：通过免疫共沉淀(Co-IP)结合质谱分析，发现THUMPD3与RNA外泌体复合物相互作用。使用THUMPD3抗体的功能实验表明，其参与核糖体RNA前体的加工，影响细胞翻译效率和应激响应。
3. **"A role for THUMPD3 in antiviral innate immunity via RNA modification"**
*Authors: Chen, L., Zhou, M., & Liu, S.*
**摘要**：研究报道THUMPD3通过修饰病毒RNA的m5C甲基化增强宿主抗病毒反应。Western blot和免疫荧光实验(基于THUMPD3抗体)显示，病毒感染后THUMPD3易位至细胞质，与干扰素信号通路关键分子相互作用。
4. **"THUMPD3 deficiency links RNA methylation to neurological disorders"**
*Authors: Gupta, A., et al.*
**摘要**：通过构建THUMPD3敲除小鼠模型，结合抗体免疫组化分析，发现THUMPD3缺失导致神经元RNA修饰异常，引发运动功能障碍和神经退行性病变，提示其在中枢神经系统中的重要作用。
(注：以上文献为示例性内容，实际引用需根据具体论文调整。)
The THUMPD3 (THUMP domain-containing protein 3) antibody is a tool used to study the THUMPD3 protein, a conserved eukaryotic enzyme implicated in RNA modification. THUMPD3 belongs to the THUMP family, characterized by a THUMP (THioUridine synthases, Methyltransferases, and Pseudouridine synthases) domain involved in tRNA processing. Specifically, THUMPD3 functions as an S-adenosylmethionine (SAM)-dependent methyltransferase, catalyzing the post-transcriptional methylation of tRNA, which is critical for stabilizing tRNA structure, ensuring translational fidelity, and regulating cellular stress responses. Dysregulation of tRNA methylation has been linked to neurological disorders and cancers, positioning THUMPD3 as a potential biomarker or therapeutic target.
The THUMPD3 antibody is typically developed in immunized animals (e.g., rabbits) using peptide antigens derived from conserved regions of the human THUMPD3 protein. It is widely utilized in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect THUMPD3 expression levels, subcellular localization (predominantly nuclear or cytoplasmic), and interactions with other RNA-modifying enzymes. Researchers employ this antibody to explore THUMPD3's role in diseases, such as its overexpression in certain cancers or its involvement in neurodevelopmental pathways. Validated antibodies ensure specificity through knockdown/knockout controls, supporting studies on RNA epigenetics ("epitranscriptomics") and expanding insights into post-transcriptional gene regulation mechanisms.
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