| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | proRS; MT-PRORS |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PARS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **PARS2抗体** 的3篇模拟参考文献及其摘要概括(基于已有研究方向的合理推测，非真实文献)：
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1. **文献名称**：*PARS2 mutations impair mitochondrial tRNA splicing and cause early-onset epileptic encephalopathy*
**作者**：Simon, M.T., et al.
**摘要**：本研究通过全外显子测序发现PARS2基因突变与婴儿期线粒体脑病相关。利用PARS2特异性抗体进行Western blot和免疫组化分析，发现突变导致PARS2蛋白表达显著降低，进而影响线粒体tRNA加工，引发神经元能量代谢障碍。
2. **文献名称**：*Mitochondrial prolyl-tRNA synthetase PARS2 deficiency links to progressive neurodegeneration*
**作者**：Chen, L., et al.
**摘要**：研究团队在小鼠模型中敲除PARS2基因，结合PARS2抗体进行脑组织免疫荧光定位，发现其在线粒体中的特异性分布。实验表明PARS2缺失导致神经元线粒体功能异常，引发海马区退行性病变，提示其与神经发育障碍的关联。
3. **文献名称**：*Development and validation of a PARS2-specific monoclonal antibody for diagnostic applications*
**作者**：Gomez, R., et al.
**摘要**：本文报道了一种新型PARS2单克隆抗体的开发，通过ELISA和免疫沉淀验证其高特异性。该抗体成功应用于临床样本检测，发现PARS2蛋白水平在神经发育异常患者中显著降低，为相关疾病的早期诊断提供了工具。
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**说明**：以上文献为模拟内容，实际研究需通过PubMed或Google Scholar检索关键词“PARS2 antibody”“PARS2 mitochondrial disease”等获取。真实文献可能更多关注基因突变机制而非抗体本身，但抗体常作为研究工具出现在方法学部分。
The PARS2 antibody is a valuable tool for detecting the PARS2 protein, a mitochondrial aminoacyl-tRNA synthetase encoded by the *PARS2* gene. This enzyme catalyzes the attachment of proline to its cognate mitochondrial tRNA, a critical step in mitochondrial protein synthesis. As part of the family of aminoacyl-tRNA synthetases, PARS2 ensures translational fidelity within mitochondria, supporting oxidative phosphorylation and cellular energy production. Mutations in *PARS2* are linked to neurodevelopmental disorders, including autosomal recessive primary epilepsy with developmental delay, highlighting its role in neuronal function.
PARS2 antibodies are widely used in research to study mitochondrial dysfunction, particularly in contexts like epilepsy, neurodegenerative diseases, and metabolic disorders. They enable protein localization via immunofluorescence, quantification through Western blotting, and tissue-specific expression analysis using immunohistochemistry. Such studies have revealed PARS2's tissue-specific expression patterns, with high levels observed in metabolically active organs like the brain, heart, and liver.
The antibody also aids in exploring the molecular mechanisms underlying *PARS2*-related pathologies, such as impaired mitochondrial translation and subsequent respiratory chain deficiencies. By facilitating the detection of PARS2 in clinical and experimental models, these antibodies contribute to understanding disease pathogenesis and evaluating potential therapeutic strategies targeting mitochondrial protein synthesis pathways.
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