| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | fibrinogen beta chain |
| Entrez GeneID | 2244 |
| clone | 1F9 |
| WB Predicted band size | 52kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human FGB (aa30-300) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于OTX1抗体的3篇参考文献示例(注：内容为模拟生成，仅供参考)：
1. **文献名称**：OTX1 expression in glioblastoma correlates with tumor malignancy and prognosis
**作者**：Smith A, et al.
**摘要**：通过免疫组化分析OTX1蛋白在胶质母细胞瘤中的表达，发现其高表达与患者生存期缩短相关，提示OTX1可能作为神经肿瘤的潜在生物标志物。
2. **文献名称**：Developmental regulation of OTX1 in the murine brain: An immunohistochemical study
**作者**：Lee C, et al.
**摘要**：利用OTX1特异性抗体研究小鼠胚胎及出生后大脑发育，发现OTX1在皮质层形成及神经前体细胞分化中具有时空特异性表达模式。
3. **文献名称**：OTX1 promotes lung cancer progression via Wnt/β-catenin signaling
**作者**：Wang Y, et al.
**摘要**：通过Western blot和免疫荧光验证OTX1在肺癌组织中的过表达，并证明其通过激活Wnt通路促进肿瘤侵袭，抗体特异性通过siRNA敲低实验验证。
(注：实际文献需通过PubMed/Google Scholar等平台检索确认。)
The OTX1 antibody targets the Orthodenticle homeobox 1 (OTX1) protein, a transcription factor belonging to the homeodomain-containing protein family. OTX1 plays critical roles in embryonic development, particularly in the central nervous system (CNS) and sensory organ formation. During neurodevelopment, it regulates the patterning and differentiation of the forebrain and midbrain. Studies also link OTX1 to retinal development and craniofacial morphogenesis. Beyond development, OTX1 is implicated in pathological processes, including cancer. Overexpression of OTX1 has been observed in tumors such as glioblastoma, medulloblastoma, and melanoma, where it may drive proliferation, invasion, and therapy resistance by modulating pathways like Wnt/β-catenin or PI3K/AKT.
OTX1 antibodies are widely used in research to study its expression, localization, and function via techniques like Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies are typically raised in rabbits or mice, with specificity validated through knockout controls or siRNA knockdown. Commercially available clones vary in host species, clonality (monoclonal/polyclonal), and epitope recognition. Researchers rely on OTX1 antibodies to explore its dual role in developmental biology and disease, particularly its potential as a diagnostic marker or therapeutic target in cancers. However, challenges remain in ensuring antibody specificity due to homology with OTX2. a closely related family member. Ongoing studies aim to clarify OTX1’s context-dependent functions and its interplay with other regulatory proteins.
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