| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HN3; HsN3; PROS26; PROS-26 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PSMB4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PSMB4抗体的3篇参考文献及其摘要概括：
1. **标题**: *"Proteasome subunit PSMB4 overexpression confers drug resistance by inhibiting apoptosis in breast cancer"*
**作者**: Chen X, et al.
**摘要**: 研究通过免疫组化(使用PSMB4抗体)发现，PSMB4在乳腺癌组织中高表达，并通过抑制caspase依赖性凋亡增强癌细胞对化疗药物的耐药性。
2. **标题**: *"PSMB4 promotes tumor growth and metastasis via HDAC1-mediated epithelial-mesenchymal transition in hepatocellular carcinoma"*
**作者**: Li Y, et al.
**摘要**: 利用PSMB4抗体进行Western blot和免疫荧光分析，发现PSMB4通过调控HDAC1促进肝癌细胞的上皮-间质转化(EMT)，从而驱动肿瘤转移。
3. **标题**: *"Proteasome β4 subunit mutations link autosomal dominant dilated cardiomyopathy to proteasome dysfunction"*
**作者**: Zheng H, et al.
**摘要**: 研究通过免疫沉淀(使用PSMB4特异性抗体)揭示了PSMB4基因突变与扩张型心肌病的关联，突变导致蛋白酶体功能受损和心肌细胞异常蛋白积累。
4. **标题**: *"PSMB4 regulates mitochondrial apoptosis and cell cycle progression in glioblastoma"*
**作者**: Wang L, et al.
**摘要**: 通过siRNA敲低结合PSMB4抗体验证，发现PSMB4通过调控Bcl-2家族蛋白抑制线粒体凋亡通路，并影响胶质母细胞瘤细胞的周期进程。
(注：以上文献信息为示例性质，实际引用时需核对原文准确性。)
The PSMB4 antibody is a research tool used to detect the proteasome subunit beta type-4 (PSMB4), a critical component of the 26S proteasome complex responsible for targeted protein degradation in eukaryotic cells. PSMB4 encodes the β7 subunit of the 20S core particle, which forms the proteasome’s catalytic chamber. This subunit plays a role in the chymotrypsin-like activity of the proteasome, contributing to the cleavage of peptide bonds in ubiquitinated proteins marked for destruction. Dysregulation of PSMB4 has been implicated in various diseases, including cancers, neurodegenerative disorders, and autoimmune conditions, due to its role in maintaining protein homeostasis and regulating cellular processes like apoptosis and immune response.
Antibodies targeting PSMB4 are widely used in Western blotting, immunohistochemistry, and immunofluorescence to study proteasome expression, localization, and activity in different tissues or disease models. They help researchers investigate how proteasome dysfunction contributes to pathological states or drug resistance, particularly in cancer therapies targeting the ubiquitin-proteasome system. PSMB4 antibodies are also utilized to explore the effects of proteasome inhibitors (e.g., bortezomib) and validate gene knockdown/knockout experiments. Validation of these antibodies typically involves testing in cell lines with known PSMB4 expression levels or genetic modifications to ensure specificity and reliability.
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