| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DRS1; RHLP; Drs1p; PP3241; HSPC259; dJ686N3.1 |
| WB Predicted band size | 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DDX27 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于DDX27抗体的相关文献摘要概括(基于公开数据模拟，仅供参考)：
1. **文献名称**: DDX27 promotes colorectal cancer growth by controlling ribosome biogenesis and protein translation
**作者**: Li S. et al. (2021)
**摘要**: 研究通过DDX27抗体进行免疫沉淀和Western blot分析，发现DDX27在结直肠癌中通过调控rRNA成熟和核糖体组装促进肿瘤增殖，其缺失导致核仁应激反应。
2. **文献名称**: DEAD-box RNA helicase DDX27 regulates stress granule dynamics during nucleolar stress
**作者**: Wang Y. et al. (2019)
**摘要**: 使用DDX27特异性抗体进行免疫荧光定位，揭示DDX27在核仁应激条件下参与细胞质应激颗粒形成，并调控mRNA翻译停滞过程。
3. **文献名称**: Systematic analysis of DEAD-box helicases reveals DDX27 as a novel regulator of 5.8S rRNA processing
**作者**: Chen Z. et al. (2017)
**摘要**: 通过抗体验证发现DDX27直接结合pre-rRNA，调控5.8S rRNA的3'端加工过程，其功能缺失导致核仁结构异常和细胞周期阻滞。
注：以上内容为基于领域知识的模拟文献，实际引用请通过PubMed/Web of Science检索最新文献。建议使用关键词"DDX27 antibody application"或"DDX27 functional study"进行精准查询。
The DDX27 antibody is a research tool designed to detect and study the DEAD-box helicase 27 (DDX27) protein, a member of the DEAD-box RNA helicase family. DDX27 is involved in various RNA-related processes, including ribosome biogenesis, RNA splicing, and translation regulation. It plays a critical role in cellular homeostasis by binding to RNA and unwinding secondary structures during RNA maturation and degradation. Dysregulation of DDX27 has been implicated in diseases such as cancer and neurodegenerative disorders, making it a target for mechanistic and therapeutic investigations.
The DDX27 antibody is typically developed in host species like rabbits or mice using immunogenic peptides or recombinant protein fragments. It enables researchers to analyze DDX27 expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Validated antibodies are essential for ensuring specificity, minimizing cross-reactivity with other DEAD-box proteins. Recent studies have utilized DDX27 antibodies to explore its role in ribosomal RNA processing and its potential as a biomarker in cancers, including colorectal and hepatocellular carcinomas.
As a reagent, the DDX27 antibody supports fundamental research into RNA metabolism mechanisms and disease pathways, aiding the development of targeted therapies. Commercial availability and validation data (e.g., species reactivity, application suitability) are critical considerations for experimental reproducibility.
×
