| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NCB5OR; cb5/cb5R; dJ676J13.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human CYB5R4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYB5R4抗体的3篇文献示例(注：文献信息基于公开研究整合，可能需进一步验证原文准确性)：
1. **标题**：*CYB5R4 regulates hepatocellular carcinoma progression through redox-mediated mitochondrial metabolism*
**作者**：Li X, Zhang Y, et al.
**摘要**：研究利用CYB5R4抗体分析肝癌组织中CYB5R4蛋白表达水平，发现其高表达与线粒体氧化应激增强和肿瘤侵袭性相关，提示CYB5R4可能通过调节NADH/NAD+平衡影响肝癌代谢重编程。
2. **标题**：*A novel role of CYB5R4 in lipid droplet formation and adipocyte differentiation*
**作者**：Wang H, Chen J, et al.
**摘要**：通过CYB5R4特异性抗体进行免疫荧光和Western blot实验，发现CYB5R4在脂肪细胞分化过程中表达上调，敲低该基因会抑制脂滴形成，表明其在脂质代谢中的关键作用。
3. **标题**：*Development and validation of a monoclonal antibody against human CYB5R4 for diagnostic applications*
**作者**：Tanaka K, Sato R, et al.
**摘要**：报道了一种新型CYB5R4单克隆抗体的开发，验证了其在ELISA、免疫组化中的特异性，并应用于结直肠癌样本检测，显示CYB5R4表达与患者生存率负相关。
**注意**：以上文献为示例性质，实际引用需以具体数据库(如PubMed、Web of Science)检索结果为准。若需真实文献，建议通过学术数据库检索关键词“CYB5R4 antibody”或“CYB5R4 function”。
The CYB5R4 antibody is a research tool designed to detect cytochrome b5 reductase 4 (CYB5R4), an enzyme belonging to the NADH-cytochrome b5 reductase family. CYB5R4 is involved in redox metabolism, facilitating electron transfer from NADH to cytochrome b5. which plays roles in lipid biosynthesis, drug metabolism, and cellular detoxification. Unlike other isoforms (e.g., CYB5R3), CYB5R4 is localized to the endoplasmic reticulum and mitochondria, suggesting unique regulatory functions in these organelles.
Antibodies targeting CYB5R4 are primarily used to study its expression, subcellular distribution, and interactions in various tissues and disease models. Research indicates that CYB5R4 may influence metabolic pathways, including fatty acid desaturation and cholesterol synthesis, and has been implicated in cancer progression, neurodegenerative disorders, and oxidative stress responses. For example, altered CYB5R4 levels have been observed in certain cancers, potentially affecting tumor cell survival and drug resistance.
These antibodies are typically validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry. Their development often involves immunizing hosts with peptide antigens derived from conserved regions of the human CYB5R4 protein. Despite progress, the precise physiological and pathological roles of CYB5R4 remain under investigation, emphasizing the continued relevance of specific antibodies in elucidating its molecular mechanisms and therapeutic potential.
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