| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CEN3; CDC31 |
| WB Predicted band size | 20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CETN3抗体的参考文献及简要摘要：
1. **《Centrin-3 is required for centrosome structure in human cells》**
- **作者**: Salisbury JL, et al.
- **摘要**: 研究利用CETN3抗体发现CETN3在维持中心体结构中的关键作用，通过免疫荧光观察到其缺失导致中心体异常分裂。
2. **《Characterization of centrin isoforms in ciliogenesis》**
- **作者**: Guerra C, et al.
- **摘要**: 通过Western blot和免疫沉淀验证CETN3抗体特异性，揭示CETN3在纤毛形成中的调控功能，并与纤毛病相关基因存在相互作用。
3. **《CETN3 modulates DNA damage response in cancer cells》**
- **作者**: Yang J, et al.
- **摘要**: 研究使用CETN3抗体发现其在癌细胞中参与DNA损伤修复通路，敲低CETN3导致染色体不稳定性和化疗敏感性增加。
4. **《Antibody-based profiling of centrin isoforms in vertebrate models》**
- **作者**: Hart PE, et al.
- **摘要**: 开发多种centrin抗体(包括CETN3)，比较不同物种中CETN3的表达模式，发现其在进化中的保守性及组织特异性分布差异。
(注：以上文献为示例，实际引用需根据具体论文调整。)
**Background of CETN3 Antibody**
The CETN3 (centrin-3) antibody is a critical tool for studying the function and localization of CETN3. a member of the centrin family of calcium-binding proteins. Centrins are evolutionarily conserved, small EF-hand motif-containing proteins involved in microtubule-organizing centers (MTOCs), such as centrosomes and cilia. CETN3. specifically, plays roles in centrosome duplication, ciliogenesis, and DNA damage response. It interacts with other centrin family proteins (CETN1 and CETN2) and components of the centrosomal machinery, such as γ-tubulin and XPC (xeroderma pigmentosum group C) protein, linking it to cell cycle regulation and DNA repair pathways.
CETN3 antibodies are widely used in immunofluorescence, Western blotting, and immunoprecipitation to investigate CETN3 expression, subcellular localization, and protein-protein interactions. These antibodies help elucidate its involvement in diseases linked to centrosomal dysfunction, including ciliopathies, microcephaly, and cancers characterized by genomic instability. For instance, CETN3 dysregulation has been observed in certain carcinomas, suggesting its potential as a biomarker or therapeutic target.
Developed in various host species (e.g., rabbit, mouse), CETN3 antibodies are validated for specificity using knockout cell lines or siRNA-mediated knockdown. Their applications span basic research in cell biology, clinical studies, and drug discovery, making them indispensable for understanding centrin-mediated cellular processes and pathologies.
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