| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HEPP; SEI-3/HEPP |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDCA4抗体的3篇参考文献示例(注：以下内容为模拟生成，文献信息可能需要进一步核实)：
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1. **文献名称**：*CDCA4 promotes hepatocellular carcinoma progression by regulating cell cycle via E2F1 signaling*
**作者**：Liu T, et al.
**摘要**：该研究通过免疫组化(使用抗CDCA4抗体)分析肝癌组织中CDCA4的表达，发现其高表达与患者预后不良相关。实验表明CDCA4通过调控E2F1靶基因驱动细胞周期进程，并利用Western blot(抗体来源：Abcam)验证了蛋白水平的变化。
2. **文献名称**：*Development of a monoclonal antibody specific to CDCA4 and its application in detecting tumor metastasis*
**作者**：Sato K, et al.
**摘要**：研究团队开发了一种高特异性的抗CDCA4单克隆抗体，并通过流式细胞术和免疫荧光验证其在乳腺癌细胞中的结合能力。实验证明，CDCA4抗体可用于检测循环肿瘤细胞中的CDCA4表达，提示其作为转移标志物的潜力。
3. **文献名称**：*CDCA4 interacts with BRCA1 to regulate DNA repair in ovarian cancer cells*
**作者**：Gupta R, et al.
**摘要**：利用抗CDCA4抗体进行免疫共沉淀(Co-IP)和质谱分析，揭示了CDCA4与BRCA1的相互作用机制。研究表明，CDCA4缺失通过抗体介导的蛋白敲低技术导致DNA修复缺陷，增强卵巢癌细胞对化疗的敏感性。
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**备注**：若需获取真实文献，建议在PubMed或Google Scholar中检索关键词“CDCA4 antibody”、“CDCA4 immunohistochemistry”或“CDCA4 protein interaction”，并筛选涉及抗体实验(如Western blot、IP、IF等)的研究。
CDCA4 (Cell Division Cycle Associated 4), also known as SEI-3 or HSPC064. is a nuclear protein implicated in regulating cell cycle progression and transcriptional activity. It belongs to the CDCA protein family, which plays roles in cellular processes such as proliferation, differentiation, and apoptosis. CDCA4 interacts with key regulators like E2F transcription factors and the retinoblastoma (Rb) protein, influencing the G1/S phase transition and DNA replication. Studies suggest its involvement in modulating p53-dependent pathways, potentially linking it to tumor suppression or oncogenesis depending on cellular context.
CDCA4 antibodies are essential tools for investigating its expression, localization, and function in both physiological and pathological conditions. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect CDCA4 in cell lines, tissues, or clinical samples. Research has explored CDCA4's overexpression in certain cancers (e.g., hepatocellular carcinoma, glioblastoma), where it may promote proliferation or chemoresistance. Conversely, its downregulation has been associated with cellular senescence. The development of specific CDCA4 antibodies enables deeper insights into its dual roles in cancer biology and normal cell cycle regulation, supporting potential applications in biomarker discovery or therapeutic targeting.
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