| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CPR4 |
| WB Predicted band size | 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CCNK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CCNK抗体的3篇参考文献示例(注：文献为假设示例，实际引用请核实真实来源)：
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1. **文献名称**: *Cyclin K regulates DNA damage response and transcriptional RNA polymerase II stability*
**作者**: Smith J, et al.
**摘要**: 本研究揭示CCNK通过调控RNA聚合酶II的磷酸化参与DNA损伤修复，利用CCNK抗体进行免疫沉淀实验，证实其与CDK12的相互作用，并证明CCNK缺失导致基因组不稳定性增加。
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2. **文献名称**: *CCNK as a potential biomarker in non-small cell lung cancer*
**作者**: Lee H, et al.
**摘要**: 通过CCNK抗体进行免疫组化分析，发现CCNK在肺癌组织中高表达，且与患者预后不良相关。研究提示CCNK可能成为肺癌治疗的靶点。
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3. **文献名称**: *Functional characterization of Cyclin K in acute myeloid leukemia*
**作者**: Garcia R, et al.
**摘要**: 利用CCNK抗体进行Western blot和流式细胞术，发现CCNK在白血病细胞中异常活化，并通过调控转录延伸促进癌细胞增殖，抑制CCNK可诱导凋亡。
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如需真实文献，建议在PubMed或Google Scholar检索关键词“Cyclin K antibody”或“CCNK function”。
CCNK (Cyclin K) is a regulatory protein belonging to the cyclin family, which plays critical roles in cell cycle progression and transcriptional regulation. It functions as a partner for cyclin-dependent kinases CDK12 and CDK13. forming complexes essential for modulating RNA polymerase II (RNAP II) activity. These complexes are involved in phosphorylation of the C-terminal domain (CTD) of RNAP II, influencing transcription elongation, mRNA processing, and DNA damage response pathways, particularly homologous recombination repair. CCNK-CDK12/13 also regulates the expression of genes associated with genome stability, including BRCA1 and other DNA repair factors.
Antibodies targeting CCNK are widely used in research to investigate its expression, localization, and interaction networks. They enable detection of CCNK protein levels in various cellular contexts, such as during cell cycle phases or under genotoxic stress. Applications include Western blotting, immunoprecipitation, and immunofluorescence to study its role in transcription-coupled DNA repair mechanisms and cell cycle checkpoints. Dysregulation of CCNK has been implicated in cancers, where genomic instability or altered CDK12/13 activity contributes to tumorigenesis. Thus, CCNK antibodies serve as tools to explore its potential as a biomarker or therapeutic target. Studies using these antibodies have advanced understanding of how transcriptional cyclins coordinate cell proliferation with stress adaptation, highlighting their relevance in cancer biology and drug development.
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