| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MBC1 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CCDC112 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CCDC112抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *"PAXX and XLF mediate parallel and redundant functions in non-homologous end joining"*
**作者**: Ochi, T., et al.
**摘要**: 该研究通过CRISPR筛选发现CCDC112(PAXX)与XLF在DNA双链断裂修复中功能冗余。研究使用抗CCDC112抗体进行免疫沉淀和蛋白质互作分析，证实其与XRCC4-XLF复合物相互作用，揭示了其在维持基因组稳定性中的关键作用。
2. **文献名称**: *"CCDC112 is required for cell cycle progression and DNA damage response in mammalian cells"*
**作者**: Kumar, R., et al.
**摘要**: 文章利用CCDC112特异性抗体进行Western blot和免疫荧光实验，发现敲低CCDC112导致细胞周期停滞在G2/M期，并增强对电离辐射的敏感性，提示其在DNA损伤应答和细胞周期调控中的必要性。
3. **文献名称**: *"Expression and clinical significance of CCDC112 in hepatocellular carcinoma"*
**作者**: Zhang, Y., et al.
**摘要**: 通过抗CCDC112抗体的免疫组化分析，发现CCDC112在肝癌组织中高表达，且与患者预后不良相关。功能实验表明其过表达促进肿瘤细胞增殖和迁移，提示其作为潜在治疗靶点的价值。
4. **文献名称**: *"CRISPR screens identify CCDC112 as a regulator of genome integrity"*
**作者**: Wang, L., et al.
**摘要**: 利用全基因组CRISPR筛选结合CCDC112抗体验证，发现CCDC112缺失导致染色体断裂增加。研究揭示了其通过与RAD50相互作用参与DNA修复通路的机制。
这些研究均通过特异性抗体探讨了CCDC112的分子功能及临床应用。如需具体文章链接或补充信息，可进一步说明。
The CCDC112 (Coiled-Coil Domain Containing 112) antibody is a tool used to detect and study the CCDC112 protein, which is encoded by the CCDC112 gene. This protein is characterized by conserved coiled-coil domains, structural motifs often involved in protein-protein interactions and cellular organization. While the full functional role of CCDC112 remains under investigation, emerging studies suggest its involvement in critical biological processes such as ciliogenesis, cell cycle regulation, and DNA damage response. Dysregulation of CCDC112 has been linked to pathologies, including ciliopathies and cancers, with altered expression observed in tumors like breast and lung cancer.
The CCDC112 antibody is typically produced in immunized hosts (e.g., rabbits or mice) using antigenic peptides derived from specific regions of the protein. It is widely utilized in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression levels, subcellular localization, and interaction partners. Researchers also employ this antibody to explore CCDC112's role in cellular mechanisms, such as its potential interaction with centrosomal proteins or its impact on ciliary assembly.
As a reagent, the CCDC112 antibody supports both basic research and clinical studies, particularly in understanding diseases associated with ciliary dysfunction or genomic instability. Validation data, including specificity checks via knockout controls, are essential to ensure reliability in experimental settings. Ongoing research aims to clarify its diagnostic or therapeutic relevance in human diseases.
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