| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EDS4A; EDSVASC; PMGEDSV |
| Entrez GeneID | 1281 |
| clone | 8C1G11 |
| WB Predicted band size | 138.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human COL3A1 (AA: 24-153) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于 MICU1 抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: "MICU1 controls cristae junction and spatially anchors mitochondrial Ca²⁺ uniporter complex"
**作者**: Patron M, et al.
**摘要**: 该研究利用MICU1抗体进行免疫沉淀和免疫荧光分析，揭示了MICU1在维持线粒体嵴连接结构及锚定MCU复合体中的关键作用，发现MICU1缺失导致线粒体钙摄取功能异常及代谢紊乱。
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2. **文献名称**: "Tissue-specific mitochondrial decoding of cytoplasmic Ca²⁺ signals is controlled by the MICU1/2 ratio"
**作者**: Kamer KJ, et al.
**摘要**: 通过Western blot和免疫组化技术结合MICU1抗体，研究发现不同组织中MICU1/MICU2表达比例差异调控线粒体钙信号敏感性，抗体实验证实MICU1高表达组织对钙摄取的阈值更高。
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3. **文献名称**: "MICU1 regulation of mitochondrial calcium uptake dictates survival and tumor progression in breast cancer"
**作者**: Tosatto A, et al.
**摘要**: 使用MICU1抗体在乳腺癌模型中发现，MICU1通过调控线粒体钙稳态影响癌细胞存活和转移，抗体敲低实验显示MICU1缺失导致线粒体钙超载并促进细胞凋亡。
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4. **文献名称**: "A MICU1 splice variant confers high sensitivity to the mitochondrial Ca²⁺ uptake machinery"
**作者**: Vecellio Reane D, et al.
**摘要**: 该研究通过MICU1抗体识别不同剪接变体，发现一种新型MICU1异构体通过改变MCU复合体构象增强钙摄取能力，抗体特异性验证为功能差异研究提供关键证据。
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这些文献均通过MICU1抗体在分子机制、疾病模型或功能验证中展开研究，涵盖结构生物学、代谢调控及癌症领域。
**Background of MICU1 Antibodies**
MICU1 (Mitochondrial Calcium Uptake 1) is a key regulatory protein involved in mitochondrial calcium homeostasis, primarily functioning as a gatekeeper for the mitochondrial calcium uniporter (MCU) complex. Located in the mitochondrial inner membrane, MICU1 forms a heterodimer with MICU2 or MICU3 to regulate calcium (Ca²⁺) influx into mitochondria. It ensures that mitochondrial Ca²⁺ uptake occurs only when cytosolic Ca²⁺ levels are sufficiently high, preventing mitochondrial Ca²⁺ overload under resting conditions. This fine-tuned regulation is critical for maintaining energy production, cellular signaling, and apoptosis.
MICU1 antibodies are essential tools for studying its expression, localization, and interactions in various tissues and disease contexts. Dysregulation of MICU1 has been linked to neuromuscular disorders, cardiovascular diseases, and metabolic syndromes. For instance, mutations in *MICU1* are associated with myopathy, cognitive impairment, and extrapyramidal symptoms. Researchers use MICU1 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore its role in mitochondrial function and pathology.
Commercially available MICU1 antibodies are typically raised against specific epitopes of human or murine MICU1. with validation in knockout models to ensure specificity. These antibodies aid in elucidating mechanisms of mitochondrial Ca²⁺ dysregulation in diseases and testing potential therapeutic strategies. Ongoing research continues to uncover MICU1's broader roles in cellular physiology, making its antibodies vital for advancing mitochondrial biology and translational medicine.
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