| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SHK; CARKL |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SHPK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与SHPK(Sedoheptulokinase)抗体相关的模拟参考文献示例(实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Development of a Monoclonal Antibody Against Human SHPK for Metabolic Disorder Diagnosis"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了一种针对人源SHPK蛋白的高特异性单克隆抗体的开发，该抗体通过ELISA和Western blot验证，成功应用于磷酸戊糖途径缺陷患者的酶活性检测，为罕见代谢疾病的诊断提供新工具。
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2. **文献名称**: "SHPK Knockout Mice Reveal Its Role in Hepatic Glucose Metabolism via Antibody-Based Tissue Staining"
**作者**: Tanaka K, et al.
**摘要**: 利用免疫组化技术结合SHPK特异性抗体，研究发现SHPK缺失小鼠肝脏中糖代谢通路异常，提示SHPK在调节肝细胞能量稳态中的关键作用，为糖尿病机制研究提供新方向。
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3. **文献名称**: "Autoantibodies Targeting SHPK in Autoimmune Encephalitis: A Case-Control Study"
**作者**: Müller S, et al.
**摘要**: 通过筛查自身免疫性脑炎患者血清，首次发现抗SHPK自身抗体的存在，并证实其与神经系统症状的相关性，为探索自身免疫疾病的生物标志物提供潜在靶点。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词“SHPK antibody”或“Sedoheptulokinase antibody”获取。建议结合具体研究背景筛选合适文献。
**Background of SHPK Antibodies**
SHPK (Sphingosine kinase) antibodies are tools used to study the role of sphingosine kinases, primarily SphK1 and SphK2. in cellular signaling and disease. Sphingosine kinases catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), a bioactive lipid regulating processes like cell survival, proliferation, immune response, and angiogenesis. Dysregulation of SphK/S1P signaling is linked to cancer, inflammation, fibrosis, and metabolic disorders.
Antibodies targeting SphK isoforms enable researchers to detect protein expression, localization, and activity in tissues or cultured cells. For example, SphK1 antibodies help explore its oncogenic role in cancers, where overexpression correlates with tumor progression and chemoresistance. SphK2 antibodies are used to investigate its context-dependent roles in apoptosis, epigenetic regulation, and metabolic diseases.
These antibodies are critical in validating experimental models (e.g., knockout cells or animal models) and assessing therapeutic targeting of SphKs. Challenges include ensuring isoform specificity, as SphK1 and SphK2 share structural homology but often exert opposing functions. Recent studies also highlight their potential as biomarkers or therapeutic targets, driving demand for high-affinity, validated SHPK antibodies in both basic research and drug development.
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