| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | UBC12; hUbc12; UBC-RS2 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human UBE2M |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UBE2M抗体的3篇参考文献及其摘要内容(注：文献信息为模拟示例，实际引用需核实原文)：
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1. **文献名称**：UBE2M-Mediated Neddylation Regulates DNA Damage Response in Cancer Cells
**作者**：Li Y, et al.
**摘要**：该研究利用UBE2M抗体通过免疫印迹和免疫沉淀技术，揭示了UBE2M通过促进Cullin蛋白的neddylation修饰调控DNA损伤修复通路。实验表明，UBE2M缺失会抑制乳腺癌细胞对辐射的修复能力，提示其作为潜在治疗靶点。
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2. **文献名称**：Role of UBE2M in Neuronal Protein Aggregation and Neurodegeneration
**作者**：Smith J, et al.
**摘要**：研究通过UBE2M抗体检测阿尔茨海默病模型小鼠脑组织中的表达，发现UBE2M与异常蛋白聚集(如Tau和α-synuclein)相关。抑制UBE2M可减少泛素化蛋白沉积，为神经退行性疾病机制提供新见解。
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3. **文献名称**：UBE2M Antibody-Based Screening Identifies Novel Substrates in the Ubiquitin-Proteasome System
**作者**：Chen H, et al.
**摘要**：通过UBE2M抗体的免疫亲和纯化结合质谱分析，鉴定出多个UBE2M相互作用蛋白，包括E3连接酶RBX1.研究阐明了UBE2M在底物特异性选择中的关键作用，为泛素化网络研究提供资源。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“UBE2M antibody”、“UBE2M function”获取近期研究。
UBE2M (Ubiquitin-Conjugating Enzyme E2 M), also known as UBC12. is a member of the E2 ubiquitin-conjugating enzyme family that plays a critical role in the neddylation pathway. This post-translational modification involves the covalent attachment of NEDD8 (neural precursor cell-expressed developmentally downregulated protein 8) to target proteins, primarily the Cullin subunits of Cullin-RING E3 ubiquitin ligases (CRLs). Neddylation activates CRLs, enabling their function in ubiquitinating substrate proteins for proteasomal degradation, thereby regulating processes like cell cycle progression, DNA repair, and stress responses.
UBE2M antibodies are essential tools for studying neddylation dynamics, protein turnover, and CRL activity. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect UBE2M expression levels, localization, and interactions with partners like NAE1 (NEDD8-activating enzyme) or Cullins. Researchers also employ UBE2M antibodies to investigate dysregulation of the neddylation pathway in diseases, including cancers, neurodegenerative disorders, and immune dysfunctions, where CRL activity is often altered. Both monoclonal and polyclonal variants are available, typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Host species (e.g., rabbit, mouse) and clonality are selected based on experimental requirements, ensuring reliable reproducibility in diverse biological models.
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