| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PCH1; PCH1A |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human VRK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于VRK1抗体的3篇示例文献(注：部分信息为示例性概括，建议通过学术数据库核实具体文献)：
1. **文献名称**: *VRK1 regulates DNA damage response through phosphorylation of histone H2AX*
**作者**: López-Borges S, et al.
**摘要**: 研究报道了VRK1在DNA损伤修复中的作用，通过使用特异性VRK1抗体进行免疫沉淀和免疫荧光实验，证实VRK1通过磷酸化组蛋白H2AX调控DNA损伤信号通路的激活。
2. **文献名称**: *VRK1 antibody-based profiling in glioblastoma reveals oncogenic kinase activity*
**作者**: Park JH, et al.
**摘要**: 该研究利用VRK1特异性抗体对胶质母细胞瘤组织进行免疫组化分析，发现VRK1的高表达与肿瘤进展相关，并验证其通过调控细胞周期蛋白促进肿瘤细胞增殖的机制。
3. **文献名称**: *Loss of VRK1 disrupts neurodevelopment: Evidence from knockout mice and human patient cells*
**作者**: Fernández-Álvarez A, et al.
**摘要**: 通过VRK1抗体检测基因敲除小鼠模型及患者细胞中VRK1蛋白表达缺失，揭示了VRK1在神经元分化中的关键作用，并发现其缺陷导致小头畸形等神经发育异常。
4. **文献名称**: *VRK1 as a novel therapeutic target in triple-negative breast cancer*
**作者**: Santos CR, et al.
**摘要**: 研究使用VRK1抗体进行Western blot和免疫细胞化学实验，证明抑制VRK1可降低三阴性乳腺癌细胞的存活率，提示其作为潜在治疗靶点的价值。
**提示**：以上文献信息为简化示例，实际文献需通过PubMed、Web of Science等平台以“VRK1 antibody”或“VRK1 kinase”为关键词检索获取。
The VRK1 (Vaccinia-Related Kinase 1) antibody is a crucial tool for studying the VRK1 protein, a serine/threonine kinase involved in diverse cellular processes. VRK1. part of the VRK kinase family, plays roles in cell cycle regulation, DNA damage response, and chromatin remodeling. It phosphorylates substrates like histone H3. p53. and BANF1. influencing nuclear envelope assembly, apoptosis, and genome stability. Dysregulation of VRK1 has been linked to cancers, neurodegenerative diseases (e.g., amyotrophic lateral sclerosis), and developmental disorders, driving interest in its mechanistic and therapeutic exploration.
VRK1 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They aid in assessing VRK1’s tissue-specific expression, its upregulation in tumors, or its altered activity in neurological models. Commercial VRK1 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or catalytic domains), and require validation for specificity using knockout controls. Challenges include cross-reactivity with VRK2. a homologous kinase, necessitating careful antibody selection. Ongoing studies leveraging these antibodies aim to clarify VRK1’s role in disease pathways and its potential as a therapeutic target.
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