| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ALFY; ZFYVE25 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human WDFY3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WDFY3抗体的3篇代表性文献的示例(请注意，以下内容为模拟示例，实际文献需通过数据库验证)：
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1. **文献名称**：*WDFY3 regulates the autophagosome formation pathway in mammalian cells*
**作者**：Sagona AP, et al.
**摘要**：该研究揭示了WDFY3蛋白通过调控自噬相关蛋白的招募，参与哺乳动物细胞中自噬体的形成机制。文中使用WDFY3抗体进行免疫共沉淀实验，证实其与PI3K复合物的相互作用。
2. **文献名称**：*Structural insights into WDFY3's role in selective autophagy*
**作者**：Tsuboyama K, et al.
**摘要**：通过冷冻电镜和WDFY3抗体的免疫荧光定位，研究解析了WDFY3蛋白的结构及其在选择性自噬(如线粒体自噬)中的功能，发现其C端结构域对底物识别至关重要。
3. **文献名称**：*WDFY3 antibody-based profiling links its expression to tumor progression*
**作者**：Jacomin AC, et al.
**摘要**：利用WDFY3抗体对多种癌症组织进行免疫组化分析，发现WDFY3在乳腺癌和胶质瘤中高表达，并与患者预后不良相关，提示其作为潜在肿瘤标志物的价值。
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**备注**：以上文献信息为基于WDFY3已知功能的模拟概括，实际引用时建议通过PubMed或Google Scholar以“WDFY3 antibody”或“WDFY3 autophagy”等关键词检索最新文献。
The WDFY3 (WD repeat and FYVE domain-containing protein 3) antibody is a tool used to detect the WDFY3 protein, a key regulator in selective autophagy. WDFY3. also known as Autophagy-Linked FYVE Protein (ALFY), contains WD40 repeats and a FYVE zinc finger domain, enabling interactions with ubiquitinated substrates and phosphatidylinositol 3-phosphate (PI3P) on autophagosomal membranes. It functions as a scaffold protein, bridging ubiquitinated cargo (e.g., damaged mitochondria or protein aggregates) to LC3-positive autophagosomes for degradation. Studies highlight its role in PINK1/Parkin-mediated mitophagy and neurodevelopmental processes.
WDFY3 antibodies are widely used in research to study autophagy mechanisms, protein localization, and expression levels via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies are often validated in knockout cell lines to ensure specificity. Dysregulation of WDFY3 has been implicated in neurodegenerative diseases (e.g., Parkinson’s) and neurodevelopmental disorders (e.g., autism spectrum disorders), linking its function to cellular homeostasis and neural development. Commercial WDFY3 antibodies are typically raised in rabbits or mice, targeting specific epitopes within its large (~400 kDa) structure. Researchers prioritize antibodies verified for cross-reactivity in their model organisms (e.g., human, mouse) to ensure experimental reliability.
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