| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HTB2; Htb2p; HTB1; Htb1p; Histone H2B.1; Histone H2B.2; SPT12;;p-Histone H2B.1 (T129) |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Yeast |
| Immunogen | A synthesized peptide derived from yeast Histone H2B.1 around the phosphorylation site of T129 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇关于RBM8A抗体的参考文献概览：
---
1. **文献名称**：*RBM8A regulates hematopoiesis through mRNA splicing and is critical for mouse embryonic development*
**作者**：Zhang Y et al.
**摘要**：研究利用RBM8A特异性抗体在小鼠模型中揭示了该蛋白通过调控mRNA剪接参与造血干细胞分化和胚胎发育。Western blot和免疫组化结果显示，RBM8A缺失导致关键造血基因剪接异常。
2. **文献名称**：*A hypomorphic allele of RBM8A causes TAR syndrome with incomplete penetrance in humans*
**作者**：Albers CA et al.
**摘要**：通过患者样本分析，结合RBM8A抗体的免疫荧光实验，发现RBM8A表达降低与TAR综合征(血小板减少-桡骨缺失综合征)相关。研究强调了RBM8A在肢体发育和血小板生成中的剂量敏感性。
3. **文献名称**：*RBM8A interacts with the oncoprotein YBX1 to promote cancer metastasis by enhancing translation of EMT-related mRNAs*
**作者**：Wang L et al.
**摘要**：利用RBM8A抗体进行免疫共沉淀(Co-IP)和蛋白质组学分析，发现RBM8A通过与YBX1相互作用增强上皮-间质转化(EMT)相关mRNA的翻译，促进肿瘤转移。研究为癌症靶向治疗提供了新方向。
---
这些文献展示了RBM8A抗体在胚胎发育、遗传疾病及癌症机制研究中的应用，涵盖基础机制与临床关联方向。
The RBM8A antibody is a tool used to detect RBM8A (RNA Binding Motif Protein 8A), a protein encoded by the *RBM8A* gene located on human chromosome 1q21.1. RBM8A, also known as Y14. is a core component of the exon junction complex (EJC), which plays a critical role in mRNA processing, including splicing, surveillance, and export. It interacts with other EJC proteins, such as MAGOH, to regulate gene expression and maintain RNA stability. RBM8A is involved in nonsense-mediated mRNA decay (NMD), a quality control mechanism that degrades transcripts with premature stop codons.
Mutations or deletions in *RBM8A* are linked to developmental disorders, notably TAR syndrome (thrombocytopenia-absent radius), characterized by blood and skeletal abnormalities. Dysregulation of RBM8A has also been implicated in cancers, such as glioblastoma and colorectal cancer, where it may influence tumor progression via aberrant RNA metabolism.
RBM8A antibodies are widely used in research to study its expression, localization, and interactions using techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate its role in RNA biology and disease mechanisms. Specificity and validation (e.g., knockout cell lines) are critical to ensure accurate detection, given its homology with other RNA-binding proteins. Understanding RBM8A's functions through antibody-based assays contributes to insights into genetic disorders and potential therapeutic targets.
×
