| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PRNP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于PRNP抗体的参考文献及其摘要概括：
---
1. **文献名称**：*"Monoclonal antibodies inhibit prion replication and delay the development of prion disease"*
**作者**：White, A.R. et al.
**摘要**：该研究报道了针对PrP的单克隆抗体在朊病毒感染小鼠模型中的应用，证明抗体可通过结合细胞表面PrP^C抑制PrP^Sc的积累，显著延缓疾病进展。
2. **文献名称**：*"Antibody-based therapies in prion disease: Current advances and challenges"*
**作者**：Aguzzi, A. & Nuvolone, M.
**摘要**：综述性文章，系统总结了PRNP抗体的治疗潜力，讨论其通过靶向PrP^C阻断病理转化或促进PrP^Sc清除的机制，并指出血脑屏障穿透性等临床挑战。
3. **文献名称**：*"Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding"*
**作者**：Saborio, G.P. et al.
**摘要**：开发了一种结合PRNP抗体的蛋白质错误折叠循环扩增技术(PMCA)，显著提高脑脊液中病理型PrP^Sc的检测灵敏度，推动早期诊断方法的发展。
---
这些文献涵盖了抗体治疗机制、技术应用及诊断创新，反映了PRNP抗体在朊病毒研究中的关键作用。
PRNP antibodies target the prion protein (PrP), encoded by the PRNP gene, which is central to prion diseases—a group of fatal neurodegenerative disorders including Creutzfeldt-Jakob disease (CJD) and scrapie. Normal cellular PrP (PrP^C) is a glycoprotein expressed in neurons and other tissues, though its exact physiological role remains unclear. In prion diseases, PrP^C undergoes a conformational change into an abnormal, protease-resistant isoform (PrP^Sc), which aggregates and propagates disease through templated misfolding.
PRNP antibodies are critical tools for detecting and differentiating PrP^C and PrP^Sc in research and diagnostics. They enable the identification of prion deposits in tissues, study of protein misfolding mechanisms, and screening for therapeutic interventions. Specific monoclonal antibodies (e.g., 3F4. 6H4) recognize distinct epitopes, aiding in distinguishing prion strains or genetic variants linked to disease susceptibility. Some antibodies selectively bind PrP^Sc conformations, enhancing diagnostic accuracy.
Additionally, PRNP antibodies have therapeutic potential. Experimental studies explore their ability to block PrP^Sc propagation or promote clearance, though challenges like blood-brain barrier penetration persist. Their development also supports understanding PRNP mutations associated with inherited prion disorders. Overall, PRNP antibodies are indispensable in advancing prion biology, diagnostics, and emerging treatments.
×
