| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NSE2; MMS21; ZMIZ7; C8orf36 |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NSMCE2抗体的参考文献示例(注：文献为示例性质，建议通过学术数据库核实具体研究内容)：
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1. **文献名称**：*NSMCE2 is essential for the integrity of the SMC5/6 complex and DNA repair*
**作者**：Taylor EM, et al.
**摘要**：
本研究通过生成针对NSMCE2的多克隆抗体，验证了其在SMC5/6复合体中的关键作用。实验表明，NSMCE2抗体能特异性识别内源性蛋白，并通过免疫沉淀证明其与复合体其他组分的相互作用。功能研究揭示了NSMCE2缺失导致DNA损伤修复缺陷。
2. **文献名称**：*The role of NSMCE2 in maintaining genomic stability through SUMOylation*
**作者**：Potts PR, et al.
**摘要**：
利用NSMCE2特异性抗体，作者发现NSMCE2通过调控SMC5/6复合体的SUMO连接酶活性参与DNA复制叉的稳定。抗体在免疫荧光实验中显示NSMCE2定位于DNA损伤位点，支持其在复制压力中的功能。
3. **文献名称**：*NSMCE2 depletion sensitizes cancer cells to ionizing radiation*
**作者**：Fujita M, et al.
**摘要**：
通过Western blot和免疫组化使用NSMCE2抗体，研究发现多种癌症细胞系中NSMCE2表达水平与放疗抗性相关。抗体验证了敲低后DNA修复蛋白(如γH2AX)的异常聚集，提示其作为潜在治疗靶点。
4. **文献名称**：*Antibody-based profiling of SMC5/6 complex subunits in human cells*
**作者**：Doyle JM, et al.
**摘要**：
本文系统比较了多种SMC5/6亚基抗体的特异性，包括NSMCE2抗体。实验证实该抗体适用于免疫印迹和免疫沉淀，并揭示了NSMCE2在不同细胞周期阶段的表达动态。
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**提示**：以上文献标题和摘要为模拟示例，实际研究需通过PubMed、Google Scholar等平台检索关键词(如“NSMCE2 antibody”或“SMC5/6 complex”)获取最新信息。
The NSMCE2 antibody targets the NSMCE2 protein (also known as NSE2 or MMS21), a critical subunit of the SMC5-SMC6 complex, a conserved multi-protein complex essential for maintaining genomic stability. This complex plays a central role in DNA repair, chromosome segregation, and replication fork restart, particularly during homologous recombination repair and telomere maintenance. NSMCE2. a SUMO ligase, facilitates post-translational modification of target proteins via SUMOylation, regulating their activity in DNA damage response pathways.
Antibodies against NSMCE2 are widely used in research to study its expression, localization, and interactions within the SMC5-SMC6 complex. They enable detection through techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in understanding its molecular mechanisms. Dysregulation or mutations in NSMCE2 are linked to genomic instability disorders, including Bloom syndrome-like phenotypes, and have been implicated in cancer progression, such as melanoma and lung adenocarcinoma.
Research using NSMCE2 antibodies has highlighted its role in suppressing replication stress and preventing aberrant recombination, offering insights into tumorigenesis and potential therapeutic targets. These tools remain vital for dissecting the interplay between genome maintenance pathways and human disease.
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