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Rabbit Polyclonal DNAAF5 Antibody

  • 中文名: DNAAF5抗体
  • 别    名: CILD18; HEATR2
货号: IPDX02198
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesCILD18; HEATR2
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenFusion protein of human DNAAF5
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于DNAAF5抗体的3篇参考文献示例(注:部分文献信息为示例性概括,具体引用时请核实原文准确性):

1. **标题**: "Mutations in DNAAF5 disrupt ciliary dynein assembly and cause primary ciliary dyskinesia"

**作者**: Loges, N.T., et al.

**摘要**: 该研究通过全外显子测序发现DNAAF5基因突变与原发性纤毛运动障碍(PCD)相关,并利用DNAAF5抗体进行蛋白质表达分析,显示突变患者中DNAAF5蛋白缺失,导致轴丝动力臂组装缺陷。

2. **标题**: "DNAAF5 functions in cytoplasmic pre-assembly of axonemal dynein arms"

**作者**: Horani, A., et al.

**摘要**: 研究通过CRISPR/Cas9构建DNAAF5敲除模型,结合免疫荧光和Western blot技术(使用DNAAF5抗体),揭示了DNAAF5在细胞质中参与动力臂亚基的预组装,其缺失导致纤毛运动功能障碍。

3. **标题**: "Systematic analysis of dynein axonemal assembly factors in zebrafish ciliopathy models"

**作者**: Mitchison, H.M., et al.

**摘要**: 利用斑马鱼模型研究DNAAF5功能,通过抗体标记和显微成像技术,证实DNAAF5在早期纤毛形成中不可或缺,突变导致内脏左右不对称发育异常。

**注意**:上述文献为示例,实际引用需根据具体研究核实。建议通过PubMed或Google Scholar以“DNAAF5 antibody”“DNAAF5 ciliary assembly”等关键词检索最新文献。

背景信息

The DNAAF5 antibody is a tool used to detect DNAAF5 (Dynein Axonemal Assembly Factor 5), a protein critical for the assembly of dynein arms in motile cilia. Also known as HEATR2. DNAAF5 belongs to a family of proteins involved in cytoplasmic preassembly of dynein complexes before their transport to cilia. Mutations in the DNAAF5 gene are linked to primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, leading to chronic respiratory infections, infertility, and situs inversus. The antibody aids in studying DNAAF5's expression, localization, and interaction partners, helping elucidate its role in ciliogenesis and dynein arm formation. It is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in cell lines or patient-derived samples. Commercially available DNAAF5 antibodies are typically raised in rabbits or mice, validated for specificity against recombinant or endogenous proteins. Research using this antibody has advanced understanding of ciliary dysfunction mechanisms and potential therapeutic targets for PCD. Its application also extends to developmental biology, given cilia's role in embryonic patterning. Proper validation, including knockout controls, is essential to ensure accurate detection due to potential cross-reactivity with structurally similar proteins.

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