| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MATS2; MOB4A; MOBKL1A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MOB1B抗体的3篇参考文献的简要概括：
1. **文献名称**: "MOB1B mediates proliferation and apoptosis in colorectal cancer via the Hippo/YAP signaling pathway"
**作者**: Li X, et al.
**摘要**: 该研究利用MOB1B抗体验证其在结直肠癌中的表达水平，发现MOB1B通过调控Hippo/YAP通路影响癌细胞增殖和凋亡，提示其作为潜在治疗靶点的可能性。
2. **文献名称**: "Characterization of MOB1B in cell cycle regulation and centrosome dynamics"
**作者**: Hergovich A, et al.
**摘要**: 研究通过免疫印迹(Western blot)和免疫荧光技术，使用MOB1B抗体揭示其在细胞周期调控中的作用，表明MOB1B缺失会导致中心体异常和有丝分裂缺陷。
3. **文献名称**: "MOB1B forms a conserved kinase complex with STK38/NDR1 and regulates neuronal morphogenesis"
**作者**: Bichsel SJ, et al.
**摘要**: 文章通过免疫共沉淀(Co-IP)实验结合MOB1B抗体，证明MOB1B与STK38激酶相互作用，调控神经元形态发生，并影响Hippo通路下游效应因子。
4. **文献名称**: "The Hippo pathway component MOB1B regulates cellular senescence through p21 signaling"
**作者**: Oh HJ, et al.
**摘要**: 研究利用MOB1B抗体进行功能缺失实验，发现MOB1B通过激活p21依赖的细胞衰老机制抑制肿瘤生长，为癌症治疗提供新思路。
这些文献均通过MOB1B抗体探讨其在信号传导、细胞周期和疾病中的功能，涵盖基础机制与潜在临床应用。
MOB1B (Mps One Binder Kinase Activator 1B) is a regulatory protein component of the Hippo signaling pathway, a conserved signaling network critical for controlling organ size, cell proliferation, apoptosis, and tissue homeostasis. It shares high sequence homology with MOB1A, its paralog, and both act as adaptor proteins to bridge interactions between upstream kinases (MST1/2) and downstream effectors (LATS1/2). MOB1B forms a complex with MST1/2. facilitating LATS1/2 activation through phosphorylation, which subsequently inhibits the oncogenic transcriptional co-activators YAP/TAZ.
Dysregulation of MOB1B has been implicated in cancer, where reduced expression or functional mutations may disrupt Hippo pathway activity, promoting uncontrolled cell growth and metastasis. Studies suggest MOB1B also participates in mitotic progression, cytokinesis, and genomic stability through interactions with NDR1/2 kinases.
MOB1B-specific antibodies are essential tools for investigating its expression, localization, and post-translational modifications in cellular contexts. These antibodies enable applications like Western blotting, immunofluorescence, and immunohistochemistry to assess MOB1B levels in disease models or clinical samples. However, due to the high similarity between MOB1A and MOB1B (~90% amino acid identity), antibody specificity must be rigorously validated using knockout controls to avoid cross-reactivity. Phospho-specific antibodies targeting MOB1B's phosphorylation sites (e.g., Thr74) further aid in studying its activation dynamics in Hippo signaling. Research utilizing these reagents continues to clarify MOB1B's dual roles in tumor suppression and cell cycle regulation.
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