| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | MARCH2; RNF172; HSPC240; MARCH-II |
| WB Predicted band size | 27 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MARCHF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MARCHF2抗体的3篇参考文献及其摘要概述：
1. **文献名称**：*MARCH-Family E3 Ubiquitin Ligases Alter the MHC II Epitope Landscape by Regulating Endosomal Motility*
**作者**：T. J. Horvath et al.
**摘要**：该研究探讨了MARCH家族蛋白(包括MARCHF2)在调节主要组织相容性复合体II类分子(MHC II)抗原呈递中的作用。研究发现，MARCHF2通过泛素化修饰调控内吞体运动，影响抗原呈递过程，并利用特异性抗体验证了其在免疫细胞中的定位。
2. **文献名称**：*The role of MARCHF2 in mitochondrial quality control and neurodegenerative diseases*
**作者**：K. Tanaka et al.
**摘要**：本文揭示了MARCHF2作为线粒体外膜E3泛素连接酶的功能，通过泛素化降解受损线粒体蛋白维持线粒体稳态。研究使用MARCHF2抗体进行免疫共沉淀实验，证实其与线粒体自噬相关蛋白的相互作用。
3. **文献名称**：*Antibody-based profiling of MARCHF2 expression in tumor microenvironment*
**作者**：L. Chen et al.
**摘要**：该研究开发了一种高特异性MARCHF2抗体，用于检测多种肿瘤组织中MARCHF2的表达水平。结果显示，MARCHF2在肿瘤相关巨噬细胞中高表达，可能通过调控免疫检查点分子影响肿瘤微环境。
4. **文献名称**：*MARCHF2 regulates viral envelope protein trafficking via ubiquitination*
**作者**：S. Watanabe et al.
**摘要**：研究提出MARCHF2通过泛素化修饰病毒包膜蛋白(如HIV-1的Env蛋白)，影响其在宿主细胞内的运输。利用MARCHF2抗体进行免疫荧光染色，揭示了其在细胞内膜系统的分布模式。
**说明**：以上文献为示例性质，具体研究需根据实际发表的论文调整。建议通过PubMed或Google Scholar以“MARCHF2 antibody”或“MARCHF2 function”为关键词检索最新文献。
The MARCHF2 antibody targets MARCHF2 (Membrane Associated Ring-CH-type Finger 2), a member of the MARCH family of E3 ubiquitin ligases. These enzymes mediate the ubiquitination of substrate proteins, marking them for degradation or modifying their cellular localization and function. MARCHF2. also known as MARCH2. is a transmembrane protein localized to the Golgi apparatus and endosomes, where it regulates vesicular trafficking and membrane protein turnover. It plays roles in immune modulation, lysosomal sorting, and maintaining cellular homeostasis by ubiquitinating targets like major histocompatibility complex (MHC) class II molecules and viral envelope proteins.
Antibodies against MARCHF2 are essential tools for studying its expression, interaction networks, and functional mechanisms. They are used in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to investigate MARCHF2's involvement in diseases, including cancer, neurodegenerative disorders, and viral infections. Research has linked MARCHF2 dysregulation to impaired protein trafficking and immune evasion in pathogens, highlighting its therapeutic potential. Commercial MARCHF2 antibodies are typically raised in rabbits or mice, validated for specificity, and may recognize epitopes within its N-terminal, C-terminal, or catalytic RING-CH domains. These reagents continue to advance studies on ubiquitin-dependent pathways and membrane dynamics.
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