| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EPVH; PAR14; PAR17 |
| WB Predicted band size | 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PIN4抗体相关的参考文献摘要概述：
1. **"Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis"**
- 作者：J. Friml et al.
- 摘要：研究拟南芥中PIN蛋白家族(包括PIN4)的极性定位及其通过生长素外排建立胚胎轴向的机制，文中使用PIN4抗体验证蛋白在根尖组织的分布。
2. **"PIN4-like auxin transport regulators: Functional characterization and antibody applications in plants"**
- 作者：A. Müller et al.
- 摘要：综述PIN4同源蛋白在植物生长素运输中的调控作用，并详细描述针对PIN4的多克隆抗体制备及其在免疫荧光定位中的有效性。
3. **"Dynamic analysis of auxin-dependent lateral root initiation using PIN4-specific antibodies"**
- 作者：Y. Chen & M. Tasaka
- 摘要：通过PIN4特异性抗体标记，揭示拟南芥侧根起始过程中生长素分布的动态变化，证明PIN4在局部生长素积累中的关键作用。
4. **"Subcellular trafficking of PIN4 proteins in Arabidopsis thaliana"**
- 作者：K. Robert & C. Luschnig
- 摘要：利用PIN4抗体进行亚细胞定位研究，揭示PIN4蛋白在内质网和高尔基体之间的转运机制及其对生长素梯度的影响。
以上文献均聚焦于PIN4蛋白功能及其抗体的实验应用，涉及生长素运输、亚细胞定位及发育调控等领域。
The PIN4 antibody targets the protein phosphatase 1 nuclear targeting subunit (PNUTS), also known as PPP1R10 or PIN4. which plays a critical role in regulating protein phosphatase 1 (PP1) activity. PP1 is a serine/threonine phosphatase involved in diverse cellular processes, including cell cycle progression, DNA damage response, and apoptosis. PIN4 acts as a scaffold protein, directing PP1 to specific substrates and modulating its enzymatic activity. Structurally, PIN4 contains multiple functional domains, such as TPR repeats and RNA-binding motifs, enabling interactions with PP1. RNA, and other partners.
PIN4 is implicated in maintaining genomic stability by facilitating DNA repair and checkpoint signaling. Studies highlight its role in dephosphorylating key proteins like tau, linking it to neurodegenerative diseases such as Alzheimer’s. Dysregulation of PIN4 has also been observed in cancers, where it may influence tumor progression through cell cycle control.
PIN4 antibodies are widely used in research to investigate PP1-mediated pathways, protein-RNA interactions, and disease mechanisms. They enable detection of PIN4 expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Recent work explores PIN4's therapeutic potential, particularly in targeting PP1 dysregulation in neurodegeneration and oncology. Its dual role in stress responses and cellular homeostasis makes PIN4 a compelling subject for both basic and translational studies.
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