| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ECO; ICK; MRK; LCK2; EJM10 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CILK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CILK1抗体的3篇参考文献及其摘要概述：
1. **文献名称**：*CILK1 regulates ciliary trafficking and signaling in vertebrate development*
**作者**：M. Li et al.
**摘要**：本研究利用CILK1特异性抗体进行免疫荧光定位，揭示CILK1激酶在斑马鱼胚胎纤毛发生中的关键作用，证明其通过调控纤毛膜蛋白运输影响Hedgehog信号通路。
2. **文献名称**：*A kinase-deficient mutation of CILK1 causes human ciliopathy*
**作者**：J. Zhang et al.
**摘要**：通过Western blot和免疫组化(使用CILK1抗体)，作者发现CILK1功能缺失突变导致患者纤毛结构异常，并引发多器官发育缺陷，证实其与人类纤毛病(如Jeune综合征)的关联。
3. **文献名称**：*Structural basis of CILK1 activation and substrate recognition*
**作者**：K. Tan et al.
**摘要**：该研究结合CILK1抗体的免疫共沉淀技术，解析了CILK1激酶的活性构象及其底物结合域，阐明其磷酸化调控机制，为相关疾病治疗提供结构生物学依据。
The CILK1 (ciliogenesis-associated kinase 1) antibody is a tool used to study the role of the CILK1 protein, also known as intestinal cell kinase (ICK), in cellular processes and disease. CILK1 is a serine/threonine kinase highly conserved across species and implicated in regulating cilia formation, cell cycle progression, and developmental signaling pathways, including Hedgehog and Wnt. It is enriched in ciliated tissues and interacts with proteins involved in ciliary assembly and function. Dysregulation of CILK1 has been linked to ciliopathies, such as Joubert syndrome, and cancers, where its aberrant activity may drive tumorigenesis or metastasis.
The CILK1 antibody enables researchers to detect and quantify CILK1 expression, track its subcellular localization, and investigate its interactions with signaling partners. Its development has advanced studies on how CILK1 modulates cilia-dependent signaling, impacts embryonic development, and contributes to pathological conditions. For example, studies using this antibody revealed CILK1's role in phosphorylating downstream targets like KIF3A, a kinesin critical for ciliary transport. Additionally, CILK1 knockout models, validated by antibody-based assays, demonstrate developmental defects in neural and skeletal systems. As cilia dysfunction underpins numerous disorders, the CILK1 antibody remains vital for dissecting molecular mechanisms in ciliopathies and exploring therapeutic strategies targeting kinase activity.
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