| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TBCA抗体的3篇文献示例(注：内容为虚构示例，仅展示格式)：
1. **文献名称**：*TBCA Antibody Characterization in Cancer Cell Models*
**作者**：Tian, L. et al.
**摘要**：研究通过制备高特异性TBCA多克隆抗体，验证其在乳腺癌细胞系中的表达水平，发现TBCA表达与微管稳定性及细胞迁移能力相关。
2. **文献名称**：*Role of Tubulin Chaperone TBCA in Neuronal Development*
**作者**：Hernández-López, R. et al.
**摘要**：利用TBCA抗体进行免疫荧光定位，揭示TBCA在小鼠神经元中的亚细胞分布，并证明其缺失导致轴突生长异常。
3. **文献名称**：*TBCA Antibody Application in Protein Interaction Studies*
**作者**：Martin, S.G. & Anders, F.
**摘要**：通过免疫共沉淀(Co-IP)结合TBCA抗体，鉴定TBCA与微管伴侣复合体其他组分(如TBCB)的相互作用机制。
(提示：实际文献需通过PubMed或专业数据库检索，以上为模拟示例。)
Tubulin-specific chaperone A (TBCA) is a critical protein involved in the folding and assembly of β-tubulin, a key component of microtubules. Microtubules are dynamic cytoskeletal structures essential for cell division, intracellular transport, and maintaining cell shape. TBCA, part of the tubulin chaperone system (including TBCB, TBCC, and TBCD), ensures proper β-tubulin folding by binding to nascent polypeptides, preventing aggregation, and facilitating their transfer to subsequent chaperones for heterodimerization with α-tubulin. This process is vital for functional microtubule formation.
Research on TBCA antibodies has grown due to their utility in studying tubulin biogenesis and microtubule-related pathologies. Antibodies targeting TBCA enable detection and quantification of the protein in cells, aiding investigations into its expression patterns, subcellular localization, and interactions. Dysregulation of TBCA is linked to neurodevelopmental disorders and cancer, as altered tubulin dynamics can disrupt mitosis or neuronal migration. For example, TBCA mutations are associated with encephalopathies and microcephaly, while its overexpression is observed in certain tumors.
TBCA antibodies are widely used in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation, providing insights into microtubule assembly mechanisms and disease pathways. Their development underscores the importance of chaperone systems in cellular homeostasis and highlights potential therapeutic targets for microtubule-associated diseases.
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