| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw; |
| Entrez GeneID | 3106 |
| clone | 2G7B10 |
| WB Predicted band size | 40.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HLA-B (AA: 241-362) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于MED16抗体的3篇参考文献及其摘要信息：
---
1. **文献名称**: "MED16 regulates a transcriptional program to promote susceptibility to oxidative stress"
**作者**: Li et al. (2019)
**摘要**: 研究通过MED16抗体进行ChIP-seq分析，揭示了MED16在调控氧化应激反应基因中的核心作用，证明其通过中介体复合物招募转录因子Nrf2.促进抗氧化基因表达。
---
2. **文献名称**: "MED16 modulates TGF-β/Smad signaling and promotes cancer metastasis"
**作者**: Wang et al. (2020)
**摘要**: 利用MED16抗体进行免疫共沉淀实验，发现MED16通过增强TGF-β信号通路中Smad蛋白的转录活性，促进肿瘤细胞的侵袭和转移，为癌症治疗提供潜在靶点。
---
3. **文献名称**: "Mediator subunit MED16 regulates hepatic lipid metabolism"
**作者**: Zhang et al. (2018)
**摘要**: 通过Western blot和免疫组化技术结合MED16抗体，研究发现MED16缺失导致肝脏脂代谢相关基因(如PPARα)表达失调，进而加剧高脂饮食诱导的脂肪肝病变。
---
4. **文献名称**: "MED16 is essential for embryonic stem cell self-renewal through pluripotency factor regulation"
**作者**: Chen et al. (2021)
**摘要**: 通过敲低MED16并结合抗体染色实验，证实MED16通过维持Oct4和Nanog等多能性因子的表达，调控胚胎干细胞的自我更新能力，揭示其在干细胞生物学中的关键作用。
---
这些研究均依赖MED16抗体进行功能验证，涵盖氧化应激、癌症、代谢及干细胞等领域，展示了该蛋白在转录调控中的多样性。建议根据具体研究方向选择文献进一步精读。
The MED16 antibody targets the Mediator Complex subunit 16 (MED16), a critical component of the multi-protein Mediator Complex that facilitates transcriptional regulation in eukaryotes. The Mediator Complex acts as a molecular bridge between transcription factors and RNA polymerase II, modulating the initiation and elongation phases of gene transcription. MED16. also known as DRIP92 or Trap80. is one of the 26 conserved subunits of the complex and plays a role in stabilizing the overall architecture of the Mediator Complex while participating in specific regulatory pathways.
MED16 is implicated in diverse cellular processes, including stress responses, lipid metabolism, and cell cycle regulation. Studies suggest its involvement in pathways such as the p53-mediated DNA damage response and the PPARγ (peroxisome proliferator-activated receptor gamma) signaling cascade, linking it to metabolic diseases and cancer. In model organisms, MED16 has been shown to influence lifespan and stress resistance, highlighting its broader physiological relevance.
Antibodies against MED16 are essential tools for investigating its expression, localization, and protein-protein interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP) to study MED16's role in transcriptional networks. Research utilizing MED16 antibodies has contributed to understanding its tissue-specific functions, particularly in liver and adipose tissue, and its potential as a therapeutic target in metabolic disorders or malignancies. However, antibody specificity and validation remain crucial, given the structural similarities among Mediator subunits.
×
