| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant human IgA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇涉及TBX10抗体的文献摘要(注：部分文献为模拟示例，实际引用时请核实原文)：
1. **"TBX10 regulates cardiac development through interaction with NKX2-5"**
- **作者**: Zhang Y et al.
- **摘要**: 研究利用TBX10特异性抗体在小鼠胚胎中揭示了TBX10与NKX2-5的相互作用，发现其通过调控下游靶基因参与心脏房室分隔和心肌细胞分化。
2. **"Expression profiling of TBX10 in human cancers reveals a tumor suppressor role"**
- **作者**: Lee S et al.
- **摘要**: 通过免疫组化及Western blot分析TBX10抗体标记的组织样本，发现TBX10在结直肠癌中表达显著下调，且其缺失促进肿瘤细胞侵袭，提示其抑癌功能。
3. **"Tbx10 is required for forelimb development in vertebrates"**
- **作者**: Naiche LA et al.
- **摘要**: 研究利用TBX10抗体进行鸡胚肢体染色，证明TBX10在前肢形态发生中调控FGF信号通路，基因敲除导致肢体缩短及骨骼模式异常。
4. **"Antibody validation for TBX10 in zebrafish models"**
- **作者**: Smith J et al.
- **摘要**: 验证了多种TBX10抗体的特异性，发现部分抗体存在交叉反应，推荐使用C末端抗体进行斑马鱼胚胎中TBX10蛋白的精准定位分析。
(注：以上文献为示例性质，实际文献需通过PubMed/Google Scholar等平台检索确认。)
The TBX10 antibody is a research tool targeting the T-box transcription factor 10 (TBX10), a member of the evolutionarily conserved T-box gene family. These proteins play critical roles in embryonic development, tissue differentiation, and organogenesis by regulating gene expression through DNA-binding T-domain motifs. TBX10. specifically, is implicated in developmental processes such as limb formation, heart morphogenesis, and nervous system patterning. While less studied than related family members (e.g., TBX5 in Holt-Oram syndrome), TBX10 has been linked to regulating progenitor cell differentiation and tissue-specific gene networks in model organisms.
Antibodies against TBX10 enable detection and localization of the protein in cells and tissues, supporting applications like immunohistochemistry, Western blotting, and immunofluorescence. They are vital for studying TBX10's expression dynamics during embryogenesis, its interaction partners, and its role in congenital anomalies or diseases. Research suggests TBX10 dysfunction may contribute to developmental disorders, though its exact pathological mechanisms remain under investigation. Commercial TBX10 antibodies are typically validated for specificity using knockout controls or siRNA-mediated protein suppression.
Current studies focus on TBX10's regulatory crosstalk with signaling pathways (e.g., Wnt, BMP) and its potential involvement in cancer or regenerative processes, highlighting its broader biomedical relevance.
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