| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship |
| WB Predicted band size | 133 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human INPP5D |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于INPP5D(SHIP1)抗体的3篇代表性文献示例，涵盖不同研究方向和抗体应用场景：
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1. **文献名称**：*INPP5D regulates amyloid-beta plaque-associated microglial reactivity in Alzheimer's disease*
**作者**：Smith A, et al.
**摘要**：本研究通过Western blot和免疫组化技术，利用INPP5D特异性抗体检测阿尔茨海默病模型小鼠脑组织中INPP5D的表达分布。结果显示，INPP5D在小胶质细胞中显著下调，且与β-淀粉样斑块周围的炎症反应密切相关，提示其可能通过调控PI3K/AKT通路影响神经退行性病变。
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2. **文献名称**：*Targeting INPP5D in tumor-associated macrophages enhances antitumor immunity*
**作者**：Chen L, et al.
**摘要**：研究采用INPP5D抗体进行流式细胞术和免疫荧光染色，分析肿瘤微环境中巨噬细胞的INPP5D表达水平。实验发现，抑制INPP5D可逆转肿瘤相关巨噬细胞的免疫抑制表型，并增强T细胞抗肿瘤活性，为癌症免疫治疗提供了新靶点。
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3. **文献名称**：*Development of a high-affinity monoclonal antibody against INPP5D for functional studies*
**作者**：Wang Y, et al.
**摘要**：本文报道了一种新型INPP5D单克隆抗体的开发与验证。通过ELISA、免疫沉淀(IP)和质谱分析，证明该抗体具有高特异性和亲和力，成功应用于检测B细胞和树突状细胞中INPP5D的磷酸化状态，为研究其调控免疫受体信号通路提供了可靠工具。
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**备注**：以上文献信息为基于领域研究的示例性总结，实际文献需通过PubMed/Google Scholar检索确认。若需具体文章，可提供研究方向(如疾病模型、技术方法)进一步筛选。
INPP5D (inositol polyphosphate-5-phosphatase D), also known as SHIP1 (Src homology 2 domain-containing inositol phosphatase 1), is a lipid phosphatase encoded by the INPP5D gene. It regulates cellular signaling by hydrolyzing phosphatidylinositol (3.4.5)-trisphosphate [PI(3.4.5)P3] to PI(3.4)P2. thereby modulating phosphoinositide-dependent signaling pathways. SHIP1 is predominantly expressed in hematopoietic cells, including macrophages, dendritic cells, mast cells, and B cells, where it acts as a negative regulator of immune receptor signaling (e.g., B cell receptors, Fc receptors) and cytokine responses. Its activity influences immune cell activation, proliferation, and survival, making it a critical player in immune homeostasis and inflammatory diseases.
INPP5D antibodies are essential tools for studying SHIP1 expression, localization, and function in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, flow cytometry, and immunoprecipitation to investigate SHIP1's role in immune regulation, cancer (e.g., leukemia, lymphoma), and neurodegenerative disorders like Alzheimer’s disease, where microglial SHIP1 has been implicated. Commercially available antibodies target specific epitopes or isoforms, with validation often including knockout controls to ensure specificity. Research involving INPP5D antibodies continues to uncover its therapeutic potential, particularly in targeting immune dysregulation or oncogenic signaling pathways.
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