| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SENP5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SENP5抗体的参考文献示例(内容基于公开研究概括，建议通过学术数据库核实具体信息)：
1. **"SENP5 regulates mitochondrial dynamics through deSUMOylation of Drp1"**
*作者：Zhang Y et al. (2018)*
摘要：本研究利用SENP5特异性抗体，通过免疫共沉淀和Western blot技术，揭示了SENP5通过去SUMO化修饰Drp1蛋白调控线粒体分裂的机制，并发现其在神经退行性疾病中的潜在作用。
2. **"Nuclear-cytoplasmic trafficking of SENP5 modulates ribosome biogenesis"**
*作者：Yun C et al. (2015)*
摘要：通过免疫荧光和亚细胞分离实验(使用SENP5抗体)，文章证明SENP5在核仁与细胞质间的转运影响核糖体生成，并参与细胞应对DNA损伤的应激反应。
3. **"SENP5-mediated deSUMOylation of Aurora B kinase ensures faithful chromosome segregation"**
*作者：Li H et al. (2013)*
摘要：研究利用SENP5抗体进行染色质免疫沉淀(ChIP)，发现SENP5通过调控Aurora B激酶的SUMO化状态，维持有丝分裂中染色体的正确分离，缺陷可能导致基因组不稳定性。
4. **"Characterization of SENP family protease specificity using domain-specific antibodies"**
*作者：Shen LN et al. (2006)*
摘要：通过开发针对SENP家族各成员(含SENP5)的特异性抗体，验证了SENP5对SUMO-2/3亚型的选择性，并阐明其在SUMO前体加工中的独特作用。
**注意**：以上为示例性内容，实际文献需通过PubMed/Google Scholar等平台以关键词“SENP5 antibody”或“SENP5 function”检索确认。
The SENP5 antibody is a research tool designed to detect and study Sentrin/SUMO-specific protease 5 (SENP5), a member of the SENP family of proteases. SENP enzymes regulate SUMO (Small Ubiquitin-like Modifier) post-translational modifications by cleaving precursor SUMO proteins to their mature forms or removing SUMO conjugates from target proteins. This dynamic process, termed SUMOylation, plays critical roles in cellular functions such as nuclear-cytoplasmic transport, chromatin remodeling, DNA repair, and stress responses. SENP5. localized predominantly in the nucleolus and mitochondria, is implicated in ribosome biogenesis, mitochondrial dynamics, and apoptosis. Dysregulation of SENP5 has been linked to diseases, including cancer and neurodegenerative disorders, underscoring its biological relevance.
SENP5 antibodies are typically generated in immunized hosts (e.g., rabbits or mice) using antigenic regions of the SENP5 protein, such as specific peptide sequences or recombinant fragments. These antibodies are validated for applications like Western blotting, immunofluorescence, and immunoprecipitation to assess SENP5 expression, localization, and interactions. Specificity is confirmed using knockout cell lines or siRNA-mediated knockdown controls. Researchers employ SENP5 antibodies to investigate its role in modulating SUMOylation dynamics under physiological or pathological conditions, such as cellular stress, tumorigenesis, or mitochondrial dysfunction. Their utility extends to mechanistic studies exploring how SENP5-mediated deSUMOylation impacts signaling pathways, genomic stability, and organelle function, offering insights into therapeutic targeting opportunities.
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