WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/20-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
Aliases | RASAL |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | Fusion protein of human RASAL1 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RASAL1抗体的3篇参考文献的简要总结:
1. **文献名称**:*RASAL1 is a potent regulator of RAS signaling in multiple cancers*
**作者**:Zhang et al.
**摘要**:研究通过Western blot和免疫组化(使用RASAL1特异性抗体)发现,RASAL1在结直肠癌中表达下调,其缺失通过激活RAS-MAPK通路促进肿瘤转移,提示其作为抑癌基因的潜在作用。
2. **文献名称**:*Epigenetic silencing of RASAL1 promotes chemoresistance in hepatocellular carcinoma*
**作者**:Wang et al.
**摘要**:利用RASAL1抗体检测肝癌组织样本,发现RASAL1启动子甲基化导致表达沉默,与化疗耐药性相关,恢复其表达可增强肝癌细胞对顺铂的敏感性。
3. **文献名称**:*RASAL1 modulates TGF-β signaling in renal fibrosis*
**作者**:Li et al.
**摘要**:在小鼠肾纤维化模型中,通过免疫荧光(使用抗RASAL1抗体)发现RASAL1表达降低,其过表达可抑制TGF-β介导的成纤维细胞活化,为干预纤维化提供新靶点。
注:以上文献为示例,实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“RASAL1 antibody” + “expression”/“cancer”/“signaling”等进一步查找近期研究。
The RASAL1 antibody targets the Ras GTPase-activating protein (RasGAP) RASAL1. a critical regulator of Ras signaling pathways. RASAL1 functions as a tumor suppressor by modulating Ras activity through its GTPase-activating domain, which accelerates the conversion of active GTP-bound Ras to its inactive GDP-bound state, thereby inhibiting downstream signaling involved in cell proliferation, survival, and differentiation. Dysregulation of RASAL1. often due to promoter hypermethylation or genetic mutations, has been implicated in various cancers, including colorectal, ovarian, and renal carcinomas, where its loss correlates with increased Ras-driven oncogenesis.
RASAL1 antibodies are essential tools for studying its expression, localization, and functional roles in both normal and pathological conditions. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cell lines. Research utilizing these antibodies has revealed RASAL1's involvement in maintaining cellular homeostasis, suppressing fibrosis, and regulating metabolic processes. Additionally, studies highlight its potential as a biomarker for disease prognosis or therapeutic targeting.
The development and validation of high-specificity RASAL1 antibodies remain crucial for advancing understanding of Ras signaling dynamics and exploring therapeutic strategies to restore RASAL1 function in cancers and other Ras-related disorders.
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