| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | A6; PTK9 |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human TWF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TWF1抗体的模拟参考文献示例(文献为虚构，仅用于示例)：
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1. **文献名称**: *TWF1 regulates actin dynamics in cancer cell migration via Arp2/3 complex interaction*
**作者**: Smith J, et al.
**摘要**: 本研究利用TWF1特异性抗体，揭示了TWF1蛋白通过调控Arp2/3复合体的活性，促进乳腺癌细胞的侵袭性迁移。实验表明，TWF1敲低后细胞伪足形成减少，且抗体阻断实验进一步验证了其功能依赖性。
2. **文献名称**: *Development of a monoclonal antibody against human TWF1 for diagnostic applications*
**作者**: Lee S, et al.
**摘要**: 报道了一种新型抗人TWF1单克隆抗体的开发与验证。该抗体在免疫组化(IHC)和蛋白质印迹(Western blot)中表现出高特异性，可用于检测肿瘤组织中TWF1的过表达，为癌症病理诊断提供潜在工具。
3. **文献名称**: *TWF1 deficiency disrupts neuronal growth cone dynamics in vitro*
**作者**: Chen R, et al.
**摘要**: 通过TWF1抗体标记和活细胞成像技术，发现TWF1缺失导致神经元生长锥的肌动蛋白骨架紊乱，影响轴突导向。研究提示TWF1在神经系统发育中具有关键作用。
4. **文献名称**: *TWF1 interacts with Hippo pathway components: Implications for organ size control*
**作者**: Gupta P, et al.
**摘要**: 使用共免疫沉淀(Co-IP)结合TWF1抗体，发现TWF1与Hippo信号通路核心蛋白YAP/TAZ存在物理相互作用，可能通过调控细胞增殖参与肝脏再生与肿瘤发生。
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(注：以上文献信息为模拟生成，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。)
TWF1 (Twinfilin-1) is an actin-binding protein belonging to the ADF/cofilin superfamily, primarily involved in regulating cytoskeletal dynamics. It modulates actin polymerization by sequestering actin monomers and interacting with actin filaments, thereby influencing cell motility, morphology, and intracellular trafficking. TWF1 contains two ADF-H domains that enable binding to both monomeric (G-actin) and filamentous (F-actin) actin, as well as a C-terminal tail implicated in subcellular localization and interactions with signaling molecules like c-Jun N-terminal kinase (JNK). Its activity is tightly regulated by post-translational modifications, including phosphorylation, which affects its actin-binding capacity and cellular distribution.
TWF1 antibodies are essential tools for studying its expression, localization, and functional roles in physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate TWF1's involvement in processes like cell migration, endocytosis, and cancer metastasis. Dysregulation of TWF1 has been associated with tumor progression, neurological disorders, and immune responses, making it a potential biomarker or therapeutic target. Commercially available TWF1 antibodies are typically validated for specificity across human, mouse, and rat samples, aiding cross-species research. Recent studies also explore its crosstalk with Rho GTPases and implications in mechanotransduction, highlighting its multifaceted role in cellular adaptation to microenvironmental cues.
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