| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Lobe; PRAS40 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human AKT1S1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于AKT1S1抗体的参考文献及其摘要概括：
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1. **文献名称**：*AKT1S1 regulates mTORC1 activity through interaction with raptor*
**作者**：H. Sancak et al.
**摘要**：该研究利用AKT1S1特异性抗体，揭示AKT1S1(PRAS40)通过与mTORC1复合体的raptor亚基结合，抑制mTOR信号通路活性，从而调控细胞生长与自噬过程。
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2. **文献名称**：*PRAS40 phosphorylation marks mTORC1 inhibition and tumor suppression*
**作者**：B.D. Manning et al.
**摘要**：通过AKT1S1抗体进行蛋白质印迹分析，发现PRAS40的磷酸化状态与mTORC1活性负相关，其在肿瘤模型中通过抑制mTOR信号发挥抑癌作用。
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3. **文献名称**：*Akt regulates cell survival via PRAS40-mediated mTORC1 signaling*
**作者**：K. Inoki et al.
**摘要**：研究使用AKT1S1抗体检测细胞应激条件下的蛋白表达，证明AKT通过磷酸化PRAS40解除其对mTORC1的抑制，从而促进细胞存活和增殖。
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以上文献均通过AKT1S1抗体验证其在mTOR信号通路中的分子机制，涵盖功能调控、疾病关联及治疗潜力。
The AKT1 substrate 1 (AKT1S1), also known as PRAS40 (Proline-Rich AKT Substrate of 40 kDa), is a regulatory protein involved in the AKT/mTOR signaling pathway, which plays a central role in cell growth, proliferation, and survival. AKT1S1 interacts with mTOR complex 1 (mTORC1) and acts as a downstream effector of AKT kinase. Phosphorylation of AKT1S1 by AKT at Thr246 disrupts its binding to mTORC1. releasing mTORC1 to activate downstream targets like S6K1 and 4EBP1. thereby promoting protein synthesis and cell growth. Conversely, under nutrient-deprived conditions, unphosphorylated AKT1S1 inhibits mTORC1 activity, linking cellular energy status to metabolic regulation.
AKT1S1 antibodies are essential tools for studying this protein’s expression, post-translational modifications, and interactions in various contexts, including cancer, diabetes, and neurodegenerative diseases. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and immunoprecipitation, aiding research into AKT/mTOR pathway dysregulation. Phospho-specific antibodies targeting Thr246 are particularly valuable for assessing AKT1S1 activation status. Aberrant AKT1S1 expression or phosphorylation is associated with tumor progression and therapeutic resistance, making it a potential biomarker or therapeutic target. Researchers use AKT1S1 antibodies to explore its role in autophagy, apoptosis, and response to mTOR inhibitors, contributing to a deeper understanding of cellular signaling networks and disease mechanisms.
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