| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | PENT; PNMTase |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PNMT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PNMT(苯乙醇胺-N-甲基转移酶)抗体相关的文献示例及简要摘要：
1. **"Production and characterization of antibodies to rat phenylethanolamine-N-methyltransferase"**
- **作者**: Wong, D.L., et al.
- **摘要**: 该研究描述了针对大鼠PNMT蛋白的多克隆抗体的制备与特异性验证，抗体成功用于免疫组化检测肾上腺髓质及脑干中PNMT的表达分布。
2. **"Stress-induced changes in adrenal PNMT expression: Immunohistochemical analysis using a novel monoclonal antibody"**
- **作者**: Kennedy, B., et al.
- **摘要**: 开发了一种高特异性单克隆抗体，用于研究慢性应激模型中肾上腺PNMT的蛋白表达变化，揭示了应激对肾上腺素合成通路的调控机制。
3. **"PNMT antibody-based detection of catecholaminergic neurons in cardiovascular disease models"**
- **作者**: Goldstein, D.S., & Hussain, R.J.
- **摘要**: 利用PNMT抗体结合免疫荧光技术，揭示了心血管疾病动物模型中交感神经末梢及肾上腺髓质PNMT的异常表达，提示其与高血压病理相关。
4. **"Validation of PNMT antibodies for human tissue: Implications for neurodegenerative disease research"**
- **作者**: Hussain, R.J., & Kvetnansky, R.
- **摘要**: 通过Western blot和质谱分析验证了商用PNMT抗体在人类脑组织中的特异性，为阿尔茨海默病等神经退行性疾病中肾上腺素能系统的研究提供工具支持。
(注：以上文献为示例性内容，实际引用需以真实发表的论文为准。)
Phenylethanolamine N-methyltransferase (PNMT) is a critical enzyme in the catecholamine biosynthesis pathway, responsible for catalyzing the conversion of norepinephrine to epinephrine (adrenaline). It is predominantly expressed in the adrenal medulla and specific regions of the brain, such as the hypothalamus and brainstem. PNMT plays a vital role in the stress response, cardiovascular regulation, and energy metabolism by modulating epinephrine levels, a key hormone and neurotransmitter in sympathetic nervous system activation.
PNMT antibodies are immunological tools designed to detect and quantify PNMT expression in tissues or biological samples. These antibodies are widely used in neuroscience, endocrinology, and pharmacology research to investigate PNMT's distribution, regulation, and involvement in diseases. For instance, altered PNMT activity has been linked to hypertension, heart failure, and neuropsychiatric disorders like depression and anxiety. Researchers utilize PNMT antibodies in techniques such as Western blotting, immunohistochemistry, and ELISA to study enzyme expression patterns under physiological or pathological conditions, including stress, hypoxia, or drug interventions. Additionally, they aid in exploring epigenetic or genetic factors influencing PNMT transcription. High-specificity PNMT antibodies are essential for distinguishing PNMT from other catecholamine-synthesizing enzymes, ensuring accurate mechanistic insights into adrenergic signaling pathways and therapeutic target development.
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