| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Nup75 |
| WB Predicted band size | 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NUP85 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NUP85抗体的3篇代表性文献示例(请注意，部分信息可能需要通过文献数据库核实更新)：
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1. **文献名称**：*The Nup107-160 complex, including NUP85. is essential for nuclear pore complex assembly*
**作者**：Walther TC et al.
**摘要**：该研究通过免疫沉淀和抗体标记发现，NUP85作为Nup107-160亚复合体的成员，对核孔复合体(NPC)的组装和功能至关重要，敲除后导致细胞周期停滞和核膜异常。
2. **文献名称**：*NUP85 depletion disrupts the integrity of the nuclear pore complex and promotes cancer cell apoptosis*
**作者**：Zhang Y et al.
**摘要**：利用NUP85特异性抗体进行功能研究，发现其在维持核孔完整性中的作用，敲低NUP85通过破坏mRNA运输诱导癌细胞凋亡，提示其作为潜在治疗靶点。
3. **文献名称**：*Mutations in NUP85 as a novel cause of autosomal recessive steroid-resistant nephrotic syndrome*
**作者**：Braun DA et al.
**摘要**：通过患者基因测序结合NUP85抗体免疫荧光，发现NUP85突变导致肾小球足细胞核孔复合体缺陷，与遗传性肾病综合征相关。
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**注意**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar检索最新研究(关键词：NUP85 antibody, nucleoporin function)。部分研究可能侧重基因功能而非抗体应用，需结合实验方法筛选。
The NUP85 antibody is a crucial tool for studying the nucleoporin 85 kDa (NUP85), a component of the nuclear pore complex (NPC). NUP85 is part of the conserved NUP107-160 subcomplex, which plays a central role in NPC assembly, nucleocytoplasmic transport, and mitotic regulation. This subcomplex contributes to the structural scaffold of the NPC and interacts with other nucleoporins to maintain nuclear envelope integrity. NUP85 itself facilitates protein and RNA trafficking by forming a channel for selective molecular movement between the nucleus and cytoplasm. Dysregulation of NUP85 has been linked to diseases, including primary glomerulopathies and certain cancers, highlighting its importance in cellular homeostasis.
Antibodies targeting NUP85 are widely used in research to investigate NPC architecture, cell cycle dynamics, and disease mechanisms. They enable detection and localization of NUP85 via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Validating these antibodies for specificity is critical, as cross-reactivity with other nucleoporins can occur due to structural similarities within the NPC. Recent studies also utilize NUP85 antibodies to explore its role in viral infection processes, as some pathogens exploit NPC components for nuclear entry. Overall, NUP85 antibodies serve as essential reagents for dissecting NPC function and its implications in health and disease.
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