| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | INA5; INFA5; leIF G; IFN-alphaG; IFN-alpha-5 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human IFNA5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPP1R12A抗体的3篇参考文献示例(注：内容基于学术背景知识整理，建议通过PubMed或Google Scholar验证具体信息)：
1. **文献名称**：*"MYPT1 (PPP1R12A) phosphorylation regulates actomyosin remodeling in vascular smooth muscle"*
**作者**：Feng Q. et al.
**摘要**：本研究利用PPP1R12A特异性抗体，通过Western blot和免疫荧光技术，揭示了其在血管平滑肌收缩中的作用，发现RhoA/ROCK通路通过磷酸化MYPT1调控肌球蛋白磷酸酶活性，影响高血压模型中的血管张力。
2. **文献名称**：*"PPP1R12A as a critical regulator of tumor cell invasion via cytoskeletal dynamics"*
**作者**：Smith J.L. et al.
**摘要**：该研究在乳腺癌细胞中采用PPP1R12A抗体进行免疫沉淀分析，发现其与肌球蛋白磷酸酶复合物的相互作用影响细胞骨架重组，敲低PPP1R12A可抑制肿瘤细胞的迁移和侵袭能力。
3. **文献名称**：*"Role of PPP1R12A in neuronal development: Insights from antibody-based localization studies"*
**作者**：Chen H. & Watanabe M.
**摘要**：通过免疫组化结合PPP1R12A抗体，发现该蛋白在神经元突触前区域富集，提示其可能参与神经递质释放调控，为神经发育障碍的机制研究提供了新方向。
**注意**：以上文献信息为模拟示例，实际引用需以具体数据库检索结果为准。
The PPP1R12A antibody targets the protein phosphatase 1 regulatory subunit 12A (PPP1R12A), also known as MYPT1 (Myosin Phosphatase Target Subunit 1). This protein is a critical regulatory component of the myosin phosphatase complex, which plays a central role in regulating smooth muscle contraction and non-muscle cell motility by dephosphorylating myosin light chains. PPP1R12A binds to the catalytic subunit of protein phosphatase 1 (PP1), directing its activity toward specific substrates. Structurally, it contains multiple functional domains, including ankyrin repeats and leucine zipper motifs, which mediate protein-protein interactions and cellular localization.
Antibodies against PPP1R12A are widely used in research to study its expression, post-translational modifications (e.g., phosphorylation at Thr-696 or Ser-668), and interactions within signaling pathways. These antibodies are essential tools for investigating cellular processes such as cytoskeletal reorganization, cell adhesion, and migration, particularly in contexts like vascular tone regulation, cancer metastasis, and neurological disorders. Western blotting, immunohistochemistry, and immunofluorescence are common applications. Dysregulation of PPP1R12A has been implicated in hypertension, cardiovascular diseases, and tumor progression, making its study clinically relevant. Researchers often validate antibody specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection of PPP1R12A isoforms.
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