| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ATLD; HNGS1; MRE11A; MRE11B |
| WB Predicted band size | 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MRE11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与MRE11抗体相关的文献概览，涵盖其在DNA修复机制及疾病中的应用：
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1. **文献名称**：*MRE11/RAD50/NBS1 Complex and Its Role in DNA Repair*
**作者**：Stracker TH, Petrini JH
**摘要**：该综述详细解析了MRE11-RAD50-NBS1(MRN)复合体的结构及其在DNA双链断裂修复中的作用，强调利用MRE11抗体进行免疫沉淀和功能研究，揭示了复合体在细胞周期调控和癌症中的重要性。
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2. **文献名称**：*MRE11 Deficiency Triggers Microsatellite Instability in Colorectal Cancer*
**作者**：Giannini G, et al.
**摘要**：研究通过免疫组化(MRE11抗体)发现结直肠癌中MRE11蛋白表达降低与微卫星不稳定性(MSI)相关，提示其表达缺失可能作为肿瘤预后的生物标志物。
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3. **文献名称**：*Antibody-Based Profiling of MRE11 in Neurodegenerative Disorders*
**作者**：Bar MC, et al.
**摘要**：利用特异性MRE11抗体分析神经退行性疾病患者脑组织，发现MRE11异常聚集与神经元DNA损伤修复缺陷相关，为疾病机制提供新见解。
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**备注**：如需获取全文，可通过PubMed或期刊官网检索标题及作者。建议结合具体研究方向筛选抗体应用场景(如WB、IHC等)。
The MRE11 antibody is a crucial tool in molecular biology research, primarily used to study the MRE11 protein—a key component of the MRN complex (MRE11-RAD50-NBS1). This complex plays a central role in DNA damage response and repair mechanisms, particularly in homologous recombination (HR) and non-homologous end joining (NHEJ). MRE11 possesses exonuclease and endonuclease activities, enabling it to process DNA double-strand breaks (DSBs) for repair. Dysregulation of MRE11 is linked to genomic instability, cancer predisposition, and disorders like ataxia-telangiectasia-like disorder (ATLD).
The antibody detects MRE11 in techniques such as Western blotting, immunofluorescence, and immunohistochemistry, aiding in the study of its expression, localization, and interactions. Researchers use it to explore how MRE11 contributes to cellular responses to ionizing radiation, chemotherapeutic agents, and replication stress. It also helps identify mutations or altered expression patterns in cancers, where MRE11 may serve as a biomarker for treatment sensitivity (e.g., PARP inhibitor response) or prognosis.
Validated MRE11 antibodies are essential for specificity, as cross-reactivity with related proteins can compromise data. Commercial antibodies are typically raised against conserved epitopes, often in rabbit or mouse hosts. Recent studies also utilize MRE11 antibodies to investigate its non-canonical roles in telomere maintenance, viral defense, and immune signaling, highlighting its multifaceted biological significance. Proper experimental controls, such as knockout cell lines, are recommended to confirm antibody reliability.
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