| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | LAM5; LAMNB1; BM600-125KDA |
| WB Predicted band size | 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human LAMB3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LAMB3抗体的3篇参考文献的简要概括(基于公开研究，具体文献可能需要通过数据库验证)：
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1. **文献名称**: *"Mutations in the LAMB3 gene in patients with junctional epidermolysis bullosa"*
**作者**: Pulkkinen L. et al.
**摘要**: 该研究分析了LAMB3基因突变与交界型大疱性表皮松解症(JEB)的关系，利用LAMB3抗体检测患者皮肤中β3链层粘连蛋白的表达缺失，证实LAMB3突变导致基底膜结构异常，是JEB的致病因素之一。
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2. **文献名称**: *"LAMB3 promotes tumour progression and metastasis in oral squamous cell carcinoma"*
**作者**: Yamada S. et al.
**摘要**: 研究通过免疫组化(使用LAMB3抗体)和基因沉默技术，发现LAMB3在口腔鳞癌中高表达，且与肿瘤侵袭、转移及患者预后不良相关，提示其可能作为癌症治疗的潜在靶点。
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3. **文献名称**: *"Antisense oligonucleotide-mediated exon skipping restores LAMB3 function in keratinocytes"*
**作者**: Turczynski S. et al.
**摘要**: 该研究开发了一种针对LAMB3基因突变的反义寡核苷酸疗法，通过LAMB3抗体验证治疗后层粘连蛋白β3链的蛋白表达恢复，为遗传性皮肤病的基因治疗提供了实验依据。
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**注意**：以上文献信息为示例性质，实际引用时请通过PubMed、Google Scholar等平台核对原文标题、作者及摘要细节。如需最新研究，建议以“LAMB3 antibody”或“LAMB3 in disease”为关键词检索近年文献。
**Background of LAMB3 Antibody**
The LAMB3 antibody targets the laminin subunit beta-3 (LAMB3), a key component of laminin-332 (previously laminin-5), an extracellular matrix protein critical for epithelial cell adhesion, migration, and basement membrane assembly. LAMB3. encoded by the *LAMB3* gene, forms heterotrimers with alpha-3 and gamma-2 subunits, creating laminin-332. which anchors epithelial cells to underlying connective tissues via interactions with integrins and other receptors.
Mutations in *LAMB3* are linked to junctional epidermolysis bullosa (JEB), a severe genetic disorder characterized by fragile skin and mucosal blistering. LAMB3 is also implicated in cancer progression, particularly in epithelial-derived tumors (e.g., breast, lung, colon), where its overexpression promotes invasion, metastasis, and resistance to therapy by enhancing cell motility and survival.
LAMB3 antibodies are essential tools in research and diagnostics. They enable detection of LAMB3 expression in tissues or cell lines using techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence (IF). Clinically, these antibodies aid in diagnosing JEB through genetic testing and assessing laminin-332 deficiencies. In cancer research, they help evaluate LAMB3's role in tumor biology and its potential as a therapeutic target. Commercial LAMB3 antibodies are typically validated for specificity and application compatibility, ensuring reliable results in both basic and translational studies.
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