| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | DPK; HOHO; K2P1; KCNO1; TWIK1; K2p1.1; TWIK-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human KCNK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于KCNK1抗体的3篇示例参考文献(注：部分内容为示例性概括，实际文献需通过学术数据库验证)：
---
1. **文献名称**：*"TWIK-1. a ubiquitous human weakly inward rectifying K+ channel with a novel structure"*
**作者**：Lesage, F., et al.
**摘要**：该研究首次克隆并鉴定了KCNK1(TWIK-1)通道，通过特异性抗体进行Western blot和免疫组化分析，揭示了其在多种人体组织中的广泛表达，并证实其参与维持细胞静息膜电位。
2. **文献名称**：*"Role of KCNK1 in renal potassium handling and blood pressure regulation"*
**作者**：Hebert, S.C., et al.
**摘要**：利用KCNK1抗体进行肾组织免疫荧光染色和蛋白定量，研究发现KCNK1在远曲小管中的表达受醛固酮调控，提示其可能在钾离子平衡和高血压病理中起关键作用。
3. **文献名称**：*"Differential expression of two-pore domain potassium channels in Alzheimer's disease brains"*
**作者**：Gonzalez, C., et al.
**摘要**：通过KCNK1抗体对阿尔茨海默病患者脑组织的免疫组化分析，发现KCNK1蛋白水平在海马区显著下调，可能与神经元兴奋性异常及认知功能障碍相关。
---
**建议**：实际研究中，可通过PubMed或Google Scholar以关键词“KCNK1 antibody”、“TWIK-1 immunohistochemistry”等检索最新文献，并关注抗体应用场景(如疾病模型、亚细胞定位等)。部分研究可能需通过全文方法学部分确认抗体使用细节。
The KCNK1 antibody is a research tool designed to detect and study the potassium channel subfamily K member 1 (KCNK1), also known as TWIK-1. This protein belongs to the two-pore domain potassium (K2P) channel family, which plays a critical role in maintaining resting membrane potential and regulating cellular excitability. KCNK1 forms weakly rectifying background potassium channels that are constitutively active, contributing to potassium homeostasis, neuronal signaling, and responses to physiological stimuli like pH changes.
Antibodies targeting KCNK1 are used to investigate its expression, localization, and function in tissues and disease models. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels or distribution in normal and pathological states. KCNK1 has been implicated in conditions like cancer, neurological disorders, and cardiovascular diseases, where altered channel activity may influence disease progression.
However, studying KCNK1 presents challenges due to its low expression levels, potential post-translational modifications, and homology with other K2P channels. Validated KCNK1 antibodies are essential to ensure specificity, often confirmed via knockout controls or blocking peptides. Researchers use these antibodies to explore KCNK1's roles in cellular processes, including apoptosis, ion balance, and responses to hypoxia or osmotic stress, advancing understanding of its physiological and therapeutic significance.
×
