| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human HSD11B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HSD11B1抗体的3篇文献参考(内容为模拟示例，非真实文献)：
1. **文献名称**：*"Tissue-specific expression of HSD11B1 in human adipose and liver: A comparative immunohistochemical study"*
**作者**：Smith A, et al.
**摘要**：本研究利用特异性HSD11B1抗体，通过免疫组化技术分析其在人脂肪组织和肝脏中的表达差异，发现脂肪组织中HSD11B1蛋白水平与肥胖程度呈正相关，提示其在局部皮质醇代谢调控中的作用。
2. **文献名称**：*"Development and validation of a novel monoclonal antibody for detecting HSD11B1 in murine models of metabolic syndrome"*
**作者**：Chen L, et al.
**摘要**：研究团队开发了一种高特异性小鼠抗HSD11B1单克隆抗体，验证其在Western blot和免疫荧光中的应用，并证明代谢综合征模型中HSD11B1在肝脏中的表达显著上调，与胰岛素抵抗相关。
3. **文献名称**：*"HSD11B1 antibody-based ELISA reveals elevated serum levels in type 2 diabetes patients"*
**作者**：Wang Y, et al.
**摘要**：通过建立基于HSD11B1抗体的ELISA检测方法，发现2型糖尿病患者血清中HSD11B1浓度显著高于健康对照组，提示其可能作为代谢异常的潜在生物标志物。
(注：以上内容为示例，实际文献需通过PubMed或学术数据库检索获取。)
The 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) antibody is a tool used to study the HSD11B1 enzyme, which plays a critical role in glucocorticoid metabolism. HSD11B1 primarily catalyzes the conversion of inactive cortisone to active cortisol, regulating local glucocorticoid levels in tissues like the liver, adipose tissue, and brain. Dysregulation of HSD11B1 is linked to metabolic disorders, including obesity, insulin resistance, and hypertension, making it a target for therapeutic research.
HSD11B1 antibodies are generated using immunogenic peptides or recombinant proteins from conserved regions of the enzyme. These antibodies enable the detection and quantification of HSD11B1 expression in various experimental models via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. They help researchers investigate tissue-specific glucocorticoid activation, enzyme localization, and alterations in disease states.
Studies using HSD11B1 antibodies have revealed its role in stress response, inflammation, and metabolic homeostasis. For instance, elevated HSD11B1 in adipose tissue correlates with obesity-related metabolic dysfunction. Such findings underscore the antibody's utility in both basic research and preclinical studies targeting HSD11B1 inhibitors. Validation through knockout controls or siRNA knockdown ensures specificity, critical for interpreting its physiological and pathological relevance.
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