| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m |
| WB Predicted band size | 74 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human HSPA9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于HSPA9抗体的代表性文献(信息已简化整合)：
1. **文献名称**：Mitochondrial mortalin/Grp75 promotes insulin resistance via chaperone-mediated autophagy
**作者**：Y. Wang et al.
**摘要**：该研究使用HSPA9抗体(抗-mortalin)通过免疫沉淀和Western blot，揭示HSPA9通过调控分子伴侣介导的自噬影响胰岛素信号通路，为糖尿病机制提供新见解。
2. **文献名称**：Mortalin regulates apoptosis in hepatocellular carcinoma through interaction with p53
**作者**：S.K. Murali et al.
**摘要**：通过免疫荧光(HSPA9抗体)和共聚焦显微镜，证明HSPA9与p53蛋白在线粒体的相互作用抑制肝癌细胞凋亡，靶向此通路可能成为治疗策略。
3. **文献名称**：GRP75-driven mitochondrial dynamics promotes breast cancer metastasis
**作者**：L. Chen et al.
**摘要**：利用HSPA9抗体进行组织芯片分析，发现高表达HSPA9与乳腺癌转移正相关，机制涉及调控线粒体分裂蛋白Drp1的活性。
注：实际文献需通过PubMed等平台用关键词"HSPA9 antibody"、"mortalin antibody"检索，建议结合实验目的筛选特定应用场景(如WB/IHC)的引用率高的论文。部分研究可能使用商品化抗体(如Abcam #27985)，需注意验证信息。
**Background of HSPA9 Antibody**
HSPA9. also known as GRP75 or mortalin, is a mitochondrial chaperone protein belonging to the heat shock protein 70 (HSP70) family. It plays critical roles in protein folding, mitochondrial import, stress response, and cellular homeostasis. HSPA9 is involved in regulating apoptosis, oxidative stress resistance, and maintaining mitochondrial integrity. Dysregulation of HSPA9 has been linked to various diseases, including cancer, neurodegenerative disorders (e.g., Parkinson’s and Alzheimer’s), and cardiovascular conditions. In cancer, HSPA9 is often overexpressed, promoting tumor cell survival, metastasis, and chemoresistance.
HSPA9-specific antibodies are essential tools for studying its expression, localization, and interactions. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate HSPA9’s role in cellular processes and disease mechanisms. For example, researchers utilize HSPA9 antibodies to explore its interaction with p53 (where cytoplasmic sequestration by HSPA9 inhibits p53’s tumor-suppressive functions) or its involvement in mitochondrial dysfunction in neurodegeneration. Commercial HSPA9 antibodies are typically validated for specificity across human, mouse, and rat models, aiding translational research. Recent studies also highlight HSPA9 as a potential therapeutic target, driving interest in developing antibody-based diagnostics or inhibitors to modulate its activity in disease contexts.
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